Activation of NOX4-NLRP3 signaling pathway in renal fibrosis of aging mice
10.19405/j.cnki.issn1000-1492.2022.07.003
- Author:
Yuli Han
1
;
Xianan Dong
1
;
Liu Yang
1
;
Xuewang Li
1
;
Yan Li
1
;
Weiping Li
1
;
Weizu Li
1
Author Information
1. Dept of Pharmacology，School of Basic Medical Sciences，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
NADPH oxidase 4;
NLRP3 inflammasome;
oxidative stress;
kidney aging;
renal fibrosis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1022-1028
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects and mechanisms of NADPH oxidase 4(NOX4) and nucleotide-binding oligomeric structural domain protein-like receptor protein 3(NLRP3) inflammasome on aging-associated renal injury in mice.
Methods:The 6, 16, 20, and 24-month-old mice were used in this study. The levels of serum creatinine(SCr) and blood urea nitrogen(BUN) were detected by the kit. Frozen sections of kidney tissue were used to detect the levels of β-Galactosidase(β-Gal) and reactive oxygen species(ROS). The pathological changes of the kidney were observed by H&E, PAS, and Masson staining. The expressions of collagen Ⅳ and NLRP3 were detected by immunohistochemistry. Western blot was used to detect the expression of related proteins in the NOX4-NLRP3 signaling pathway in kidney tissues.
Results:The results showed that, compared with 6-month-old mice, the levels of BUN and SCr in serum, β-Gal activity, and ROS level in the renal cortex increased, the glomerular and tubular injury was mild, and there was no obvious renal fibrosis change in 16-month-old mice. However, in 20 and 24-month-old mice, these indexes increased, the damage of glomeruli and renal tubules increased, and renal fibrosis appeared. In addition, expression of NOX4 and NLRP3 inflammasome-associated proteins mediating ROS production was upregulated in the kidneys of 20-and 24-month-old mice.
Conclusion:The NOX4-NLRP3 signaling pathway may activate and promote renal aging and renal fibrosis during aging.
- Full text:2025022616354494516NOX4-NLRP3信号通...小鼠肾脏纤维化中的激活研究_韩玉立.pdf
