Absorption Characteristics of Nine Phenylpropanoids in Mongolian Medicine Tabson-2 Decoction in Caco-2 Cells
10.13748/j.cnki.issn1007-7693.20221847
- VernacularTitle:蒙药塔布森-2中9种苯丙素类成分在Caco-2细胞中的吸收特性研究
- Author:
LI Chunyan
1
;
WANG Xiyue
1
;
LU Jingkun
1
;
DONG Xin
1
;
ZHAO Pengwei
1
;
MA Feixiang
1
;
XUE Peifeng
1
Author Information
1. Inner Mongolia Medical University, Hohhot 010110, China
- Publication Type:Journal Article
- Keywords:
Tabson-2 decoction;
phenylpropanoids;
Caco-2 cells;
UPLC-MS/MS;
P-glycoprotein
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(15):2048-2055
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the absorption characteristics of phenylpropanoids of Mongolian medicine Tabson-2 decoction(TBD) in Caco-2 cells and to preliminarily clarify the oral absorption mechanism of TBD. METHODS Caco-2 cell monolayer model was used to analyze the uptake components of TBD in Caco-2 cells by UPLC-MS/MS, and UPLC-MS/MS analysis method was established to determine the nine best absorbed components of TBD, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptogenic acid, 1,5-dicaffeinate quinic acid, isochlorogenic acid C, caffeic acid, dihydrocaffeic acid, chlorogenic acid. The effects of time, concentration and P-glycoprotein inhibitor on the absorption of each component were investigated. RESULTS The overall intake of caffeic acid and dihydrocaffeic acid showed an upward trend in 0-180 min, and did not show saturation. The absorption of 3-hydroxycinnamic acid was constant at about 90 min and tended to saturation. The intakes of cryptochlorogenic acid, 1,5-dicaffeinate, quinic acid, isochlorogenic acid C, neochlorogenic acid, chlorogenic acid and protocatechuic acid first decreased and then increased with time from about 90 min. The addition of P-glycoprotein inhibitor verapamil and cyclosporin A had an effect on the absorption of dihydrocaffeic acid compared with the phenylpropanoid components, indicated that dihydrocaffeic acid was the substrate of P-glycoprotein. CONCLUSION The main phenylpropanoids of TBD enter Caco-2 mainly by passive diffusion, supplemented by active transport, and the absorption process of the other eight components is not affected by the efflux of P-glycoprotein except dihydrocaffeic acid.
