Q-Marker Pediction Analysis of Aurantii Fructus Immaturus in Famous Classical Formula Wendan Decoction Based on Fingerprint and Network Pharmacology
10.13748/j.cnki.issn1007-7693.20222713
- VernacularTitle:基于指纹图谱和网络药理学预测经典名方温胆汤中枳实的质量标志物
- Author:
LI Hangfei
1
;
ZHU Zhijun
1
;
LI Mingming
1
;
WANG Wanyi
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Journal Article
- Keywords:
Wendan decoction;
Aurantii Fructus Immaturus;
fingerprint;
network pharmacology;
quality marker
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(16):2216-2223
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To predict the quality marker(Q-Marker) of Aurantii Fructus Immaturus in Wendan decoction (WDD) based on fingerprint and network pharmacology. METHODS Establish the fingerprint of Aurantii Fructus Immaturus and WDD reference samples, use Chinese Medicine Chromatographic Fingerprint Similarity Evaluation Software(2012A Edition)for similarity analysis and common component identification; use network pharmacology to screen the related targets and key pathways of common components, and establish the composition-target-pathway network. Predict the potential Q-marker of expectorant stop vomiting, clear gallbladder and stomach of Aurantii Fructus Immaturus in WDD, and determine its content. RESULTS The fingerprints of 15 batches of Aurantii Fructus Immaturus and 15 batches of WDD reference samples were established, and the similarity of fingerprints between 15 batches of Aurantii Fructus Immaturus and WDD reference sample >0.9. Five chemical components were identified, which were naringin, hesperidin, neohesperidin, nobiletin and tangerine. Network pharmacologic screen analysis to getTP53, MAPK8, EGFR, JUN, MMP9 and other 32 core targets of the five compounds and Endocrine resistance, pathways in cancer, and 10 other key pathways. Construct the "component-target-pathway" network diagram. It was predicted that naringin, hesperidin, neohesperidin, nobiletin and tangerine were Q-markers of Aurantii Fructus Immaturus in WDD. The contents of naringin, hesperidin, neohesperidin, nobiletin, tangerine in WDD were not less than 0.011 7%, 0.025 3%, 0.025 7%, 0.010 4%, 0.010 7%. CONCLUSION Q-Marker prediction analysis of Aurantii Fructus Immaturus in WDD provides a reference for establishing a complete WDD quality evaluation system, and also lays a foundation for elucidating the mechanism of its pharmacodynamic material basis.
