Study on the effect of berberine combined with fluconazole on fluconazole-tolerant Candida albcians strains
10.12206/j.issn.2097-2024.202409047
- VernacularTitle:小檗碱与氟康唑合用抗氟康唑耐受白念珠菌的研究
- Author:
Zecheng SONG
1
;
Shanshan MA
1
;
Qiaoling HU
2
,
3
;
Hua ZHONG
1
;
Yan WANG
2
,
4
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China
3. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
4. The Center for Fungal Infectious Diseases Basic Research and Innovation of Medicine and Pharmacy, Ministry of Education, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
Candida albicans;
berberine;
fluconazole;
drug combination;
drug tolerance
- From:
Journal of Pharmaceutical Practice and Service
2025;43(2):87-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the combined effect of berberine （BBR） and fluconazole （FLC） on FLC-tolerant Candida albicans in vitro. Methods The sensitivity of 8 strains of Candida albicans to FLC was assessed by determining their minimal inhibitory concentration （MIC） using broth microdilution method. FLC-tolerant strains were screened from FLC-sensitive strains by disk diffusion assay. The effect of BBR combined with FLC on FLC-tolerant Candida albicans was investigated by disk diffusion assay. Results All eight strains of Candida albicans exhibited sensitivity to FLC, with minimal inhibitory concentration （MIC50） values below 0.5 μg/ml. Strains Y0109, 9821, 7879, 7654, and 9296 displayed colony growth in the inhibition zone after 48 h of constant temperature incubation, indicating FLC tolerance. When strains Y0109 and 9821 were subjected to a combination of BBR and FLC, the number of colonies within the inhibition zone decreased progressively with the increase of BBR concentration following a 48 h constant temperature culture. The inhibition zone became clear with the increasing of BBR concentration and increased with the increase of FLC loading, which showed a dose-dependent relationship. Conclusion The BBR combined with FLC demonstrated efficacy against FLC-tolerant strains.
