Optimization of purification process and component analysis of alkaloids from Zanthoxylum bungeanum Maxim
10.12206/j.issn.2097-2024.202404066
- VernacularTitle:花椒生物碱富集纯化工艺优化及其成分分析
- Author:
Heying YANG
1
;
Caiping LUO
2
;
Ting PENG
2
;
Wenyi LIANG
2
;
Songzhang SHEN
2
;
Juan SU
2
,
3
Author Information
1. School of Medicine, Shanghai University, Shanghai 200444, China.
2. National Demonstration Center for Experimental Military Pharmacy Education, Naval Medical University, Shanghai 200433, China.
3. School of Medicine, Shanghai University, Shanghai 200444, China
- Publication Type:Originalarticles
- Keywords:
Zanthoxylum bungeanum Maxim;
alkaloids;
macroporous resin;
orthogonal design;
UPLC-Q-TOF-MSE
- From:
Journal of Pharmaceutical Practice and Service
2025;43(2):75-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the process conditions and analyze the components of alkaloids from Zanthoxylum bungeanum Maxim（Z. bungeanum）using macroporous resin. Methods Combining single factor tests and orthogonal tests, the content of hydroxy-α-sanshool（HAS）and hydroxy-β-sanshool（HBS）were considered as indexes to determine the best process parameters. Ultra-performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometry（UPLC-Q-TOF-MSE）was used to identify the structures of alkaloids. Results The optimal conditions were Mitsubishi HP-20 macroporous resin, the loading solution concentration was 0.2 g crude drug/ml, the ratio of crude drug to resin volume was 1 g∶2.5 ml, the diameter/height ratio of resin column was 1∶7, the dynamic adsorption flow rate was 4 times of bed volume（BV）per hour, and the adsorption time was 1 h. Impurities were removed by using 2 BV of 20% ethanol, 5 BV of 80% ethanol was used to elution, and the content of HAS and HBS was 4.71% and 1.02%, respectively. A total of 20 alkaloids were identified from Z. bungeanum. Conclusion This method was stable and feasible, obtaining high purity and various kinds of alkaloids, which could be used for the enrichment and purification of alkaloids from Z. bungeanum.
