Preparation and Evaluation of Reactive Oxygen Species/Glutathione Dual Responsive Paclitaxel Prodrug Nanoparticle
10.13748/j.cnki.issn1007-7693.20222849
- VernacularTitle:活性氧/谷胱甘肽双重响应型紫杉醇前药纳米粒的制备与评价
- Author:
WANG Changhai
1
;
ZHANG Xinyu
1
;
JIAO Yuwen
1
;
GUO Mingxue
1
;
ZHAO Yueying
1
;
ZHANG Zekang
1
;
DU Shouying
1
;
WANG Jinling
1
;
U Yang
1
Author Information
1. Beijing University of Chinese Medicine, Beijing 100105, China
- Publication Type:Journal Article
- Keywords:
tumor microenvironment;
nanoparticle;
paclitaxel;
ROS-response;
GSH-response;
cytotoxicity
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(17):2414-2426
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To design paclitaxel prodrug nanoparticles with dual reactive oxygen species/glutathione response (ProPTX-SS-NPs), providing new ideas and methods for the application of paclitaxel. METHODS The optimal preparation method and process of prodrug nanoparticles was investigated by using particle size and PDI as indicators; the morphology of prodrug nanoparticles was observed through electron microscopy and their particle size, potential, encapsulation efficiency, drug loading capacity, etc were investigated; the in vitro release characteristics of nanoparticles in reactive oxygen species and glutathione environments were investigated; the in vitro cytotoxicity and cellular uptake of prodrug nanoparticles were investigated through cell experiments. RESULTS The nano particle size prepared by the optimal process was (130.20±2.18)nm, with the PDI of 0.12±0.01, the Zeta potential of (-8.45±0.01)mV, the drug load of (10.27±1.36)%, and the encapsulation rate of (93.22±2.20)%. The prodrug nanoparticles showed reactive oxygen species and glutathione dual responsive release ability, and could significantly inhibit the proliferation of MCF-7, HepG2, and MDA-MB-231. Its inhibitory effect on MDA-MB-231 cells was the most significant. The IC50 of prodrug nanoparticles on MDA-MB-231 cells was (0.71±0.11)μmol·L, while the IC50of PTX was (22.38±3.27)μmol·L. CONCLUSION ProPTX-SS-NPs have excellent tumor microenvironment response performance and significant anti-tumor activity, which is a highly potential and promising anti-tumor nanosystem.
