Determination, Antioxidant Activity and Reaction Kinetics of Eriodictyol and Luteolin in Xerochrysum Bracteatum
10.13748/j.cnki.issn1007-7693.20222374
- VernacularTitle:蜡菊中圣草酚和木犀草素含量测定、抗氧化活性及其反应动力学特性
- Author:
TANG Zhaoxing
1
;
WANG Miaomiao
1
,
2
;
TIAN He
1
;
YAN Huan
1
,
3
;
Ayiguli Tashibolati
1
;
LI Muchun
1
,
2
Author Information
1. Xinjiang Academic Institute of Analysis and Testing, Plant Resources Green Processing Engineering Technology Research Center of Xinjiang, Urumqi 830011, China
2. State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China
3. College of Public Health, Xinjiang Medical University, Urumqi 830046, China
- Publication Type:Journal Article
- Keywords:
Xerochrysum bracteatum;
eriodictyol;
luteolin;
content determination method;
antioxidant activities
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(18):2550-2555
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method for the determination of eriodictyol and luteolin in Xerochrysum braeteatum, and to evaluate their antioxidant activities in vitro. METHODS UPLC was used to investigate the coutent determination methodologically. Vitamin C and Trolox were used as the control, the IC50 values of eriodictyol and luteolin for scaving DPPH free radical were determined, and the antioxidant activity was compared. The kinetic characteristics of free radical scavenging reaction were preliminarily explained by the change of free radical scavenging rate within 4 h. RESULTS There was a good linear relationship (R=0.999 7) between the peak area and the concentration of eriodictyol in the range of 0.005 4-0.545 0 mg·mL-1 and luteolin in the range of 0.005 3-0.535 0 mg·mL-1. The precision, stability and recovery of the method were satisfactory. The scavenging ability of the 4 compounds from high to low was luteolin>eriodictyol>vitamin C>Trolox. Different from vitamin C and Trolox, the free radical scavenging process of eriodictyol and luteolin showed three stages:first fast and then slow. CONCLUSION The content determination method is simple and rapid, and can be used for the determination of eriodictyol and luteolin in Xerochrysum braeteatum. The resources of Xerochrysum braeteatum are worth further development because of their excellent antioxidant activities.
