Chemical Constituents of Endophytic Fungi Trichoderma sp. SHJN1 and Perenniporia sp. SHJG1 from Lindera glauca and Their Anti-tumor Activities
10.13748/j.cnki.issn1007-7693.20230437
- VernacularTitle:山胡椒内生真菌Trichoderma sp. SHJN1和Perenniporia sp. SHJG1的抗肿瘤活性成分研究
- Author:
RUAN Qingfeng
1
,
2
;
LU Qiping
3
;
CHEN Xiaojing
3
;
ZHAO Min
3
;
CUI Hui
3
;
ZHAO Zhongxiang
3
Author Information
1. Department of Pharmacy, Wuhan No. 1 Hospital, Wuhan 430022, China
2. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
3. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
- Publication Type:Journal Article
- Keywords:
Lindera glauca;
endophytic fungi;
Trichoderma sp.;
Perenniporia sp.;
secondary metabolites
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(18):2535-2542
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To find lead compounds with potent bioactivities from endophytic fungi Trichoderma sp. SHJN1 and Perenniporia sp. SHJG1 from the traditional Chinese medicine Lindera glauca. METHODS The fermentation of Trichoderma sp. SHJN1 and Perenniporia sp. SHJG1 were isolated and purificated by repeated silica gel, ODS, Sephadex LH-20, and semi-preparation HPLC. The structures of compounds were performed by NMR and ESI-MS. The preliminary evaluation of anti-tumor activity of these compounds was performed by human breast cancer cells(MCF-7) and human lung cancer cells(A549). RESULTS A total of 12 compounds were purified from the above two endophytic fungi and identified as alantrypinone(1), oryzalactam(2), phomoindene A(3), cis-gregatin B(4), huaspenone B(5), stigmasta-7,22-dien-3β,5α,6α-triol(6) and ergosterol(7), 1-deoxy-2-demethylviridiol (8), viridiol(9), trichodermamides A(10), chromone(11) and 4-hydroxyphenylacetic acid(12). The evaluation of antitumor activity showed that, compound 3 showed inhibitory activity on MCF-7 cell line with an IC50 value of (62.9±1.02)μmol·L-1[cisplatin(DDP) IC50=(30.1±1.67)μmol·L-1], while compound 8 and 9 showed inhibitory activities on A549 cell line with IC50 values of (34.6±1.57)μmol·L-1 and (44.9±1.74)μmol·L-1, respectively[DDP IC50=(20.6±1.42)μmol·L-1]. CONCLUSION Compounds 3, 8 and 9 show potent anti-tumor effects.
