Mechanism of depression-related dry eye based on 5-HT/TRPV4/AQP5
10.3980/j.issn.1672-5123.2025.3.06
- VernacularTitle:基于5-HT/TRPV4/AQP5探讨抑郁相关干眼的发病机制
- Author:
Ying YANG
1
;
Jiazhi ZHANG
1
;
Huijie JI
1
;
Yuanchen DING
1
;
Weiping GAO
1
Author Information
1. Department of Ophthalmology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
- Publication Type:Journal Article
- Keywords:
depression-related dry eye;
tear secretion;
5-HT/TRPV4/AQP5
- From:
International Eye Science
2025;25(3):372-377
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the mechanism of reduced tear secretion in depression-related dry eye rats based on 5-HT/TRPV4/AQP5.METHODS:Healthy SD male rats were established with chronic unpredictable mild stress(CUMS)method to establish a depression-induced dry eye model(n=8), and the control group was blank rats(n=8). The ELISA method was used to compare the 5-hydroxytryptamine(5-HT)in serum and hippocampal tissue of the two groups, and the HE sections of lacrimal gland and AQP5 immunohistochemistry were observed. Western blot and RT-PCR were used to detect the expression of 5-HT3R, TRPV4 and AQP5 in the lacrimal gland tissue of the two groups of rats.RESULTS:The tear secretion in the depression-induced group was significantly reduced(P=0.001), the serum and hippocampal 5-HT levels were significantly reduced(all P<0.05), the expression of AQP5 antibody in the lacrimal gland immunohistochemistry was significantly lower than that in the control group(P<0.001), the expression of 5-HT3R, TRPV4 and AQP5 in the lacrimal gland was significantly reduced(all P<0.05), and no obvious inflammatory cells were found in the lacrimal gland tissue sections.CONCLUSION:Depression-related dry eye may occur through a non-inflammatory 5-HT/TRPV4/AQP5 mechanism.
