The Beneficial Effect of Korean Red Ginseng on Rat Bladder Contractility and Oxidant Damage Following Ischemia/Reperfusion.
- Author:
Ju Hyun SHIN
1
;
Tae Hoon OH
;
Seung Woo YANG
;
Chang Shik YOUN
;
Young Ho KIM
;
Eu Gene HWANG
;
Yong Woong KIM
;
Seung Mo YUK
;
Jae Sung LIM
;
Ki Hak SONG
;
Chong Koo SUL
;
Yong Gil NA
Author Information
1. Department of Urology, School of Medicine Chungnam National University Hospital, Daejeon, Korea. synthia2000@naver.com
- Publication Type:Original Article
- Keywords:
Bladder;
Ischemia;
Reperfusion;
N-acetylcysteine
- MeSH:
Acetylcysteine;
Animals;
Aorta, Abdominal;
Carbachol;
Constriction;
Ischemia;
Lipid Peroxidation;
Luminescence;
Malondialdehyde;
Oxidative Stress;
Oxygen;
Panax*;
Rats*;
Reactive Oxygen Species;
Reperfusion;
Superoxides;
Urinary Bladder*
- From:Journal of the Korean Continence Society
2009;13(2):142-151
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: N-acetylcysteine (NAC) is a potent antioxidant, and a free radical scavenger. We investigated the possible effects of NAC after ischemia/reperfusion (I/R) of rat bladder. MATERIALS AND METHODS: I/R injury was induced by abdominal aorta clamping and ischemia for 60minutes, followed by 120minutes reperfusion. Twenty rats were divided into four groups: sham operation + saline group (S+S), sham operation + NAC group (S+NAC), I/R + saline group (I/R+S), I/R + NAC group (I/R+NAC). Blood levels of reactive oxygen species (ROS) were determined using the free oxygen radical tests (FORT). Superoxide generation was measured based on lucigenin-enhanced chemiluminescence. The level of malondialdehyde (MDA) was analyzed in order to measure lipid peroxidation. RESULTS: In I/R+S group, the isometric contractile responses to carbachol were significant lower than other groups and were reversed by the pretreatment with NAC. The level of FORT and MDA showed a marked increase in I/R+S group compared with S+S group. NADPH-stimulated superoxide production was also significantly increased. I/R+NAC decreased these parameters compared with I/R+S group. CONCLUSION: Our results suggest that treatment with NAC reversed the low contractile responses of rat bladder and prevented oxidative stress following I/R.
