Analysis of plasma metabonomic characteristics of type 2 diabetes mellitus patients with turbid toxin accumulation syndrome
10.3969/j.issn.1006-2157.2025.01.005
- VernacularTitle:2型糖尿病浊毒内蕴证患者血浆代谢组学特征分析
- Author:
Ziqi ZHAO
1
,
2
;
Pai PANG
1
,
2
;
Yue REN
1
,
2
;
Bin WANG
1
,
2
;
Yuntao MA
1
,
2
;
Qianjing YANG
1
,
2
;
Shentao WU
1
,
2
Author Information
1. First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
2. National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion
- Publication Type:Journal Article
- Keywords:
type 2 diabetes mellitus;
turbid toxin accumulation syndrome;
metabolomics;
plasma biomarkers;
metabolic pathway
- From:
Journal of Beijing University of Traditional Chinese Medicine
2025;48(1):34-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the plasma metabonomic characteristics of patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome.
Methods:One hundred and three patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome were enrolled from November 2023 to February 2024 in the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine and 54 healthy individuals were recruited. The general data of the two groups were analyzed, and the plasma metabolite content was detected using ultra-high performance liquid chromatography-Orbitrap mass spectrometry. Construct an orthogonal partial least squares discriminant analysis model to screen metabolites with significant intergroup changes. The variable importance in projection≥ 1, |log2FC|>1, and P<0.05 were used as the criteria for the screening of differential metabolites. Annotate differential metabolites using internal databases and the human metabolome database, and perform pathway analysis using MetaboAnalyst website.
Results:There was no statistically significant difference in gender and age between the two groups.Seventeen potential differential metabolites were identified. The D-4′-phosphopantothenate, 2, 6-dichloroindophenol, 4-methylphenol, hypoxanthine, 11, 12-epoxyeicosatrienoic acids, oleamide, 3-phenyllactic acid contents were higher in patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome than in healthy individuals(P<0.05); 3-anisic acid, 3-iodo-octadecanoic acid, mebendazole, β-alanine, citric acid, trans-aconitic acid, geranyl diphosphate, lysophosphatidylcholine(18∶2), phosphatidylethanolamine(18∶1), and caprolactam contents were lower in patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome than in healthy individuals(P<0.05). Ten metabolic pathways were identified, including the key metabolic pantothenate and coenzyme A biosynthesis pathways.
Conclusion:Metabolic differences were observed between patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome and healthy individuals, and the underlying mechanism may involve the pantothenate and coenzyme A biosynthesis pathways, jointly mediated by D-4′-phosphopantothenate and β-alanine.
- Full text:2025022116021636335Analysis of plasma metabonomic characteristics of type 2 diabetes mellitus patients with turbid toxin accumulation syndrome.pdf
