Dapagliflozin regulates high glucose treated endothelial progenitor cell function through AKT/eNOS pathwayDapagliflozin regulates high glucose treated endothelial progenitor cell function through AKT/eNOS pathway
10.19405/j.cnki.issn1000-1492.2022.06.021
- Author:
Dandan Xie
1
;
Tingting Wu
1
;
Xiaotong Zhao
1
;
Murong Xu
1
;
Mingwei Chen
1
Author Information
1. Dept of Endocrinology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
- Publication Type:Journal Article
- Keywords:
dapagliflozin;
diabetes mellitus;
endothelial progenitor cell;
AKT;
eNOS
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):957-962
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of dapagliflozin(DAPA) on the function of rat endothelial progenitor cells(EPCs) culturedin vitroin a high glucose environment.
Methods:Bone marrow derived EPCs from sprague-dawley(SD) rats were identified by fluorescence staining. EPCs were divided into control group(CG group), high glucose group(HG group), high glucose + DAPA group(GD group) and high glucose + DAPA + LY294002 group(GDL group). MTT assay, flow cytometry, tubule formation assay were used to detect the viability, apoptosis, tubule formation ability of EPCs, respectively. Western blot was used to detect the protein expression of AKT/eNOS signaling pathway.
Results:Compared with CG group, cell viability, the ability to form tubules, the protein expression of p-AKT and p-eNOS, and the ratio of p-AKT/AKT and p-eNOS/eNOS in HG group significantly decreased(P<0.05), while the apoptosis rate of EPCs significantly increased(P<0.05). Compared with HG group, cell viability, the ability to form tubules, the protein expression of p-AKT and p-eNOS, and the ratio of p-AKT/AKT and p-eNOS/eNOS in GD group significantly increased(P<0.05), while the apoptosis rate of EPCs was significantly reduced(P<0.05). Compared with GD group, cell viability, the ability to form tubules, the protein expression of p-AKT and p-eNOS, and the ratio of p-AKT/AKT and p-eNOS/eNOS in GDL group significantly decreased(P<0.05), while the apoptosis rate of EPCs significantly increased(P<0.05).
Conclusion:DAPA can protect EPCs from high glucose induced functional damage through AKT/eNOS pathway.
- Full text:2025022115300431679达格列净通过丝氨酸_苏氨酸...高糖环境下的内皮祖细胞功能_解丹丹.pdf
