The effect of bisphenol A on cell apoptosis pattern in the spinal cord of chick embryos.
- Author:
Tai Yang PARK
1
;
Ji Young CHA
;
Se Kyoung CHOI
;
Tae Il CHO
;
Jeong Yuen KIM
;
Kwang Jun KIM
Author Information
1. Department of Obstetrics and Gynecology, Sungae hospital, Kwang myung, Korea. xodidsun@hanmail.net
- Publication Type:Original Article
- Keywords:
Chick embryo;
Bisphenol A;
Neural tube
- MeSH:
Animals;
Apoptosis;
Benzhydryl Compounds;
Cell Death;
Chick Embryo;
Eggs;
Embryonic Structures;
Neural Tube;
Neurons;
Ovum;
Phenols;
Spinal Cord;
Wings, Animal
- From:Korean Journal of Perinatology
2008;19(3):277-282
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To investigate the effects of bisphenol A (BPA) on cell death pattern in neuronal development of chick embryos. MATERIALS AND METHODS: We planned to compare the cytokinetic features in the normal chick embryo and those with BPA. Fifteen eggs were divided into three GROUPS: the control group, BPA 50 microgram/g egg group and BPA 200 microgram/g egg group. Embryos were incubated for 56 hours (Hamburger & Hamilton stage 16) and then we injected BPA into embryos. The embryos were sectioned by 3 micrometer thickness at the level of wing buds and stained at 72 hours after incubation (HH stage 18). We observed cell death in the spinal cord using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method. RESULTS: The TUNEL-positivity markedly increased in proportion to the doses of BPA. The number of TUNEL-positive cells per section was 15.2+/-2.14 in the control group, 34.6+/-3.44 in the BPA 50 microgram/g egg group, 87.6+/-4.32 in the BPA 200 g/g egg group. Furthermore the contour of spinal cord was deformed as the doses of BPA raised. CONCLUSION: BPA causes neuronal cell death and exerts cytotoxic effect on early chick embryos. It suggests that BPA might have an effect on cytogenesis during neural tube development.
