Mechanism of ANGPTL4 regulating the migration and invasion of human lung adenocarcinoma PC9/GR cells
10.19405/j.cnki.issn1000-1492.2022.06.011
- Author:
Yue Fang
1
;
Xuan Li
1
;
Hao Cheng
1
;
Jiqing Hao
1
Author Information
1. Dept of Oncology, The First Afiliated Hospital of Anhui Medical University, Hefei 230022
- Publication Type:Journal Article
- Keywords:
non⁃small cell lung cancer;
EGFR⁃TKI resistance;
human angiopoietin⁃like protein 4;
epithelial⁃mes⁃ enchymal transition
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):902-907
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To investigate the effect of human angiopoietin-like protein 4(ANGPTL4) on the invasion and migration of gefitinib-resistant lung adenocarcinoma strains and its mechanism.
Methods:The expression of ANGPTL4 in PC9/GR cells was interfered by siRNA method, and the interference effect was verified by Western blot and qRT-PCR. The CCK-8 experiment was performed to verify the inhibitory effect of interfering with ANGPTL4 on the proliferation of PC9/GR cells. The scratch experiment and transwell experiment were performed to detect the invasion and metastasis ability of cells; the expression levels of Vimentin, E-cadherin and N-cadherin were detected by Western blot experiment.
Results:The expression of ANGPTL4 in PC9/GR cells was significantly higher than that in PC9 cells. The CCK-8 experiment proved that the IC50of PC9/GR cells to gefitinib was higher than that of PC9 cells, and the IC50was significantly reduced after interfering with the expression of ANGPTL4 in PC9/GR cells; scratch experiment and Transwell migration experiment showed that after interfering with the expression of ANGPTL4 in PC9/GR cells, the wound healing ability, migration and invasion ability of the cells were significantly reduced. The results of Western blot experiments showed that the expression of N-cadherin and Vimentin decreased and the expression of E-cadherin increased in PC9/GR cells after interference.
Conclusion:ANGPTL4 may participate in the acquired drug resistance of gefitinib in lung adenocarcinoma, and promote the invasion and metastasis of PC9/GR cells. Interference with ANGPTL4 can inhibit the EMT process of PC9/GR cells.
- Full text:2025022111022246611ANGPTL4调控人肺腺癌...R细胞迁移和侵袭机制的研究_方乐.pdf
