Isolation, culture and functional verification of primary coronary endothelial cells from macaca mulattas
10.19405/j.cnki.issn1000-1492.2022.06.010
- Author:
Haifeng Jiang
1
;
Zhen Xu
1
;
Lei Zhang
1
;
Xuewen Tan
1
;
Weile Chen
1
;
Tingyu Dong
1
;
Xiaoyi Liu
1
;
Shangxue Yan
1
;
Yan Chang
1
;
Wei Wei
1
Author Information
1. Institute of Clinical Pharmacology, Anhui Medical Uniersity, Key Lab of Anti-inflammatory andImmune Medicine , Ministry of Education, Anhui Collaborative Innovation Center of Anti-inflammatoryand Immune Medicine.Hefei 230032
- Publication Type:Journal Article
- Keywords:
macaca mulattas;
coronary artery;
primary endothelial cells;
isolation and culture;
identification;
cell modelmacaca mulattas;
coronary artery;
primary endothelial cells;
isolation and culture;
identification;
cell model
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):870-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for isolation and culture of primary endothelial cells from non-human primate coronary arteries, and to provide a cell model for the study of human coronary endothelial cells.
Methods:The coronary arteries of macaca mulattas were separated aseptically. The primary endothelial cells were separatedviatissue adhesion after collagenase digestion. CD31 positive cells were detected and sorted by flow cytometry to determine the purity of endothelial cells. After stimulation with prostaglandin E2(PGE2), the cellular viability and proliferation ability of primary coronary endothelial cells from macaca mulattas were evaluated by high-content cell imaging and CCK-8 assay, and the migration ability and tube function of primary coronary endothelial cells from macaca mulattas were measured by Transwell method and Matrigel glue method, respectively.
Results:The confluence percentage of primary coronary artery cells of macaca mulattas was about 80% after 10-14 daysin vitroculture, and the cellular morphology was irregular polygons and paver shape. The purity of endothelial cells was about 31.7% by flow cytometry. After sorting, the purity of endothelial cells was confirmed by flow cytometry, which was more than 95%. PGE2could significantly up-regulate the proliferation, migration and tube formation abilities of primary coronary endothelial cells of macaca mulattas.
Conclusion:This study successfully established the isolation and culture method of primary coronary endothelial cells from macaca mulattas, and proved that it could be used as anin vitrocell model to simulate human coronary endothelial cells through functional studies.
- Full text:2025022110495576316猕猴冠状动脉原代内皮细胞分离和培养及其功能验证_蒋海峰.pdf
