Effects of eriodictyol on the proliferation and apoptosis of triple-negative breast cancer MDA-MB-231 cells via c-Myc/AR axis
10.19405/j.cnki.issn1000-1492.2022.06.009
- Author:
Hancheng Liu
1
;
Huiming Li
1
;
Jie Zhang
1
;
Qinglai Meng
1
;
Shi Ding
2
;
Lihui Ma
1
Author Information
1. Dept of Breast Surgery, Afiliated Hospital of Chengde Medical College , Chengde 067020
2. Dept of Pharmacology, Chengde Medical College , Chengde 067000
- Publication Type:Journal Article
- Keywords:
eriodictyol;
triple⁃negative breast cancer;
proliferation;
apoptosis;
c⁃Myc;
androgen recepter
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):891-896
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To explore the effect of eriodictyol(Eri) on the proliferation and apoptosis of triple-negative breast cancer MDA-MB-231 cells and its possible mechanism.
Methods:MDA-MB-231 cells were treated with different concentrations of Eri. MTT method was used to detect the level of cell proliferation. Plate clone method was used to detect the the level of cell clonality. The apoptosis level was detected by flow cytometry. Western blot was used to detect protein expression levels of Cleaved-caspase-3, Bax, Bcl-2, c-Myc and AR. MDA-MB-231 cells were transfected with si-c-Myc and then combined treatment with 50 μmol/L Eri. MTT method was used to detect the level of cell proliferation. The apoptosis level was detected by flow cytometry. Western blot was used to detect protein expression levels of c-Myc and AR.
Results:Eri inhibited the proliferation activity of MDA-MB-231 cells in a dose and time-dependent manner. Treatment with different concentrations of Eri reduced cloning ability of MDA-MB-231 cells, promoted cell apoptosis, up-regulated the protein expression levels of Cleaved-caspase-3 and Bax, and down-regulated the protein expression level of Bcl-2, c-Myc and AR. Knockdown of c-Myc expression inhibited MDA-MB-231 cells growth, promoted cell apoptosis, and down-regulated the protein expression level of c-Myc and AR, while combined treatment with 50 μmol/L Eri could not enhance the situation.
Conclusion:Er inhibits proliferation and induces apoptosis in triple-negative breast cancer MDA-MB-231 cells, which may be achieved by inhibiting c-Myc/AR axis.
- Full text:202502211030026569圣草酚通过调控c-Myc_...231细胞增殖和凋亡的影响_刘汉成.pdf
