Improvement of inflammation and glucolipid metabolism in adipose tissues of obese mice by Mushroom
10.19405/j.cnki.issn1000-1492.2022.06.008
- Author:
Yi Chen
1
,
2
;
Binbin Zhu
1
,
2
;
Mingxuan Zheng
1
;
Fenfen Sun
1
;
Yue Zhang
1
;
Yongjia Liu
1
,
2
;
Yinghua Yu
1
,
2
;
Wei Pan
1
;
Xiaoying Yang
1
Author Information
1. Jiangsu Key Laboratory of Immunity and Metabolism , Dept of Pathogen Biology and Immunology , Xuzhou Medical University , Xuzhou 221004
2. First Clinical School of Medicine , Xuzhou Medical University , Xuzhou 221004
- Publication Type:Journal Article
- Keywords:
obesity;
Mushroom;
adipose inflammation;
glycolipid metabolism
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):885-890
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the ameliorative effects of Mushroom on adipose tissue inflammation and glucolipid metabolism in mice fed a high-fat diet, and to provide a theoretical basis for the mechanisms of Mushroom regulating glucolipid metabolism and inflammatory responses.
Methods:C57 BL/6 J mice were fed with normal diet(LF) group, high-fat diet(HF)group and high-fat diet + Mushroom(HF+Mushroom) group for 15 weeks.Then, body weight subcutaneous and epididymal white adipose tissue weight were measured. The morphological changes of adipose tissues were compared by HE staining, and the expression of genes related to inflamation, glycolysis and fatty acid oxidation pathways were detected by RT-PCR and Western blot.
Results:Compared with the LF group, the HF group had increased body weight, increased subcutaneous and epididymal white fat weight and adipocyte size, and upregulated expression of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), monocyte chemoattractant protein-1(MCP-1), CD68, inducible nitric oxide synthase(iNOS), pyruvate kinase(PK), phosphofructokinase(PFK), hypoxia inducible factor-1α(HIF-1α) and peroxisome proliferator activated receptor alpha(PPARα) in adipose tissues, while the expression of carnitine palmitoyl transferase-1 A(CPT-1 A), cytochrome P450 4 a10(CYP4 a10) and medium-chain acyl-coenzyme a dehydrogenase(MCAD) were downregulated(P<0.05). Compared with the HF group, Mushroom supplementation reduced body weight, adipose tissue weight and adipocyte size, and downregulated the expression of pro-inflammatory factors and glycolytic pathway-related factors in adipose tissues, while the expression of fatty acid oxidation pathway-related factors were upregulated(P<0.05).
Conclusion:Mushroom can ameliorate inflammation and disorders of glycolipid metabolism in adipose tissues of obese mice.
- Full text:2025022110241177755香菇多糖对肥胖小鼠脂肪炎症和糖脂代谢的改善作用_陈溢.pdf
