Differential expression of lncRNA SH3BP5-AS1 in hepatocellular carcinoma and its mechanisms of promoting hepatocarcinogenesis
10.19405/j.cnki.issn1000-1492.2022.06.006
- Author:
Dong Xu
1
;
Huanhua Lu
1
;
Xiaoliang Wang
1
;
Weixin Wu
1
Author Information
1. Qingpu Branch of Zhongshan Hospital , Fudan University, Shanghai 201700
- Publication Type:Journal Article
- Keywords:
lncRNA SH3 BPs-AS1;
hepatocellular carcinoma;
'TM6SF2;
cell proliferation;
cell apoptosis;
cell cycle
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):872-877
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To investigate the differential expression of lncRNA SH3BP5-AS1 in hepatocellular carcinoma and the possible mechanism of promoting hepatocarcinogenesis.
Methods:The expression of SH3BP5-AS1 in hepatocellular carcinoma tissue/paraneoplastic tissue(n=91) was detected by qRT-PCR. TCGA database visualization software UALCAN analyzed SH3BP5-AS1 expression in a large sample of hepatocellular carcinoma tissues(n= 371). The expression of SH3BP5-AS1 was detected by qRT-PCR in normal hepatocytes THLE-2 and hepatoma cell lines Huh 7, BEL-7405, SNU-387, Hep 3 B. After silencing SH3BP5-AS1 expression by siRNA technology, the effects of silencing SH3BP5-AS1 on the proliferation of Hep 3 B cells were detected by CCK-8 assay, cell clone formation assay and Edu assay, and the effects of silencing SH3BP5-AS1 on apoptosis and cycle of Hep 3 B cells were detected by flow-through assay. Based on the expression of SH3BP5-AS1 and TM6 SF2 in hepatocellular carcinoma tissues, the expression correlation was counted and analyzed.
Results:SH3BP5-AS1 was highly expressed in hepatocellular carcinoma tissues. SH3BP5-AS1 was highly expressed in a large sample of hepatocellular carcinoma tissues from the TCGA database SH3BP5-AS1 was highly expressed in different hepatocellular carcinoma cell lines Huh 7, BEL-7405, SNU-387 and Hep 3 B to a different extent. Silencing of SH3BP5-AS1 by siRNA inhibited the proliferation ability of Hep 3 B cells, promoted apoptosis ability, affected the cell cycle and increased the number of G1 phase cells. SH3BP5-AS1 and TM6 SF2 expression were negatively correlated in hepatocellular carcinoma tissues.
Conclusion:SH3BP5-AS1 is an important oncogenic non-coding RNA that can provide a theoretical basis for tumour-targeted therapy in patients with hepatocellular carcinoma.
- Full text:2025022110091094827lncRNA_SH3BP5...差异表达及促进肝癌发生机制_徐东.pdf
