Resarch of IL-6 in liver regeneration after APAP-induced acute liver injury
10.19405/j.cnki.issn1000-1492.2022.06.005
- Author:
Fuqian Yu
1
;
Shasha Song
1
;
Yan Lu
1
;
Lijiu Zhang
1
Author Information
1. Dept of Gastroenterology , The Second Afiliated Hospital of Anhui Medical University , Hefei 230601
- Publication Type:Journal Article
- Keywords:
acetaminophen;
acute liver injury;
interleukin⁃6;
IL⁃6 neutralizing antibody;
liver regeneration
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):866-871
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To explore the role of interleukin(IL)-6 in the process of liver regeneration after acetaminophen(APAP)-induced acute liver injury(ALI) throughin vitroexperiments.
Methods:In vitroexperiments, AML12 cell lines which originated from normal mice hepatocytes were cultured and treated with different concentrations of APAP drugs and IL-6 neutralizing antibody(IL-6 Ab). CCK-8 method was used to detect cell viability and screen out the appropriate drug effects concentration. The IL-6 concentration was measured by ELISA. Western blot was used to detect the expression levels of liver regeneration-related proteins such as PCNA, CyclinD1, and HGF. The mRNA levels of IL-6, PCNA and CyclinD1 were measured by qRT-PCR. Datas were analyzed by GraphPad Prism 8.0 software.
Results:Based on the CCK-8 data, the optimal drug concentration of 5 mmol/L APAP was selected for modeling and 0.01 μg/ml IL-6 Ab acting cells. The IL-6 concentration was 0, 1.794, 2.264, 1.658, 1.086 pg/ml at 0, 4, 12, 24, 48 h, respectively, measured by ELISA kit. Western blot results showed that compared with other time points after APAP administration on AML12 cells, the protein levels of CyclinD1 was the highest at 4 h, PCNA and HGF were the highest at 12 h(P<0.05). Compared with the 4 h APAP group, the expression of CyclinD1 and p-STAT3 protein in the APAP+IL-6 Ab group decreased(P<0.05); compared with the 12 h APAP group, the expression of PCNA and HGF in the APAP+IL-6 Ab group decreased(P<0.05). The results of qRT-PCR showed that the mRNA levels of IL-6, PCNA, and CyclinD1 in the 4 h APAP group reached the peak(P<0.05);compared with the 4 h APAP group, the PCNA and CyclinD1 levels in the APAP+IL-6 Ab group were reduced(P<0.05).
Conclusion:IL-6 Ab inhibits STAT3 phosphorylation by neutralizing IL-6 and subsequently inhibits liver regeneration. It is speculated that IL-6 may play a role in promoting liver regeneration after APAP-induced liver injury.
- Full text:2025022110022498531IL-6在对乙酰氨基酚肝损伤后再生中的作用_郁福乾.pdf
