Effects of palmitic acid sodium exposure for different time on renal tubular epithelial cells injury and NLRP3 inflammasome activation
10.19405/j.cnki.issn1000-1492.2022.06.001
- Author:
Jie Zhang
1
;
Yanhua Zhang
1
;
Yunfeng Zhu
1
;
Yan Li
1
;
Weizu Li
1
;
Weiping Li
1
Author Information
1. Dept of Pharmacology, Basic Medicine College , Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
palmitic acid sodium;
human renal tubular epithelial cells;
NLRP3 inflammasome;
reactive oxygen species;
NADPH oxidase 4
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):841-846
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To explore the effect and mechanism of palmitic acid sodium(PA) exposure for different time on HK-2 cells injury.
Methods:CCK-8 assay was used to detect the activity of HK-2 cells treated with PA at different times; oil red staining was used to detect lipid deposition at different time of PA treatment; Annexin-Ⅴ-FITC/PI apoptosis kit was used to detect the apoptosis of HK-2 cells treated with PA at different times; reactive oxygen species(ROS) production was detected by ROS kit at different time of PA treatment; ELISA was used to detect GSH and SOD in cells as well as the levels of IL-1β and IL-18 in the cell supernatant; Western blot was used to determine the expressions of NLRP3, ASC, Caspase-1 and NOX4 in HK-2 cells.
Results:CCK-8 assay showed that HK-2 cell viability decreased in PA(6, 9, 12, 24 h) groups; oil red staining showed that lipid deposition increased in PA(6, 9, 12, 24 h) groups; Annexin-Ⅴ-FITC/PI apoptosis kit results showed that the apoptosis rate increased; ROS kit results showed that ROS production significantly increased in PA(6, 9, 12, 24 h) groups; ELISA results showed that the activities of GSH and SOD decreased in PA(6, 9, 12, 24 h) groups, while the levels of IL-1β and IL-18 in supernatant increased; Western blot results showed that the expression levels of inflammatory related proteins NLRP3, Caspase-1, ASC and NOX4 in PA(6, 9, 12 and 24 h) increased.
Conclusion:PA exposure can induce lipid deposition and HK-2 cells damage, and the mechanism may be related to PA-induced inflammatory response by activating NLRP3 inflammasome in HK-2 cells.
- Full text:2025022109000498172棕榈酸钠暴露不同时间对肾小...LRP3炎症小体激活的影响_张洁.pdf
