Qualitative Analysis of Components of Glandular Hairs in Lonicera Japonica Thunb. Buds by Laser Microdissection Combined with HPLC-TOF/MS
10.13748/j.cnki.issn1007-7693.20230022
- VernacularTitle:激光显微切割技术联合HPLC-TOF/MS定性分析金银花花蕾腺毛成分
- Author:
TANG Huimin
1
;
FU Jing
1
;
XIONG Lewen
1
;
ZHANG Longfei
1
;
ZHANG Yongqing
1
;
WANG Lingna
1
Author Information
1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China
- Publication Type:Journal Article
- Keywords:
Lonicera japonica Thunb.;
glandular hair;
laser microdissection technology;
HPLC-TOF/MS;
component
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(18):2499-2505
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To accurately analyze the components of glandular hairs in Lonicera japonica Thunb. buds, and provide reference for quality evaluation and control of Lonicera japonica Thunb.. METHODS Laser microdissection technology was used to obtain glandular hairs of Lonicera japonica Thunb. buds, low-temperature freezing vortex method was used to removal flower buds surface hairs(glandular hairs and non glandular hairs), and the components of flower buds, flower buds removed surface hairs and glandular hairs were detected by HPLC-TOF/MS. Through comparison with standards, database retrieval and mass spectrometry fragmentation, the components were analyzed and identified. RESULTS Thirty-seven components were identified in the flower buds, 31 components were identified in the flower buds removed surface hairs, and 35 components were identified in the glandular hairs. Compared with flower buds, glandular hairs lack 2 phenolic acids, and flower buds removed surface hairs lack 6 flavonoids. CONCLUSION The glandular hair, which contains many secondary substances, is an important place for the synthesis and accumulation of secondary substances in Lonicera japonica Thunb. buds, and is closely related to the quality of Lonicera japonica Thunb..
