Effects of TRIM59 gene silencing on proliferation and cell cycle of colorectal cancer cells
10.19405/j.cnki.issn1000-1492.2022.05.023
- Author:
Xiaobin Gao
1
;
Xueliang Wu
1
;
Shengjie Wang
1
;
Guangyuan Sun
1
;
Wenjing Wang
1
;
Feng Liang
1
;
Yifeng Zhao
1
;
Zhenxian Liu
1
Author Information
1. Dept of General Surgery, First Afiliated Hospital of Hebei North University, Zhangjiakou 075000
- Publication Type:Journal Article
- Keywords:
TRIM59;
colorectal cancer;
cell proliferation;
cell cycle
- From:
Acta Universitatis Medicinalis Anhui
2022;57(5):796-801
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of tripartite motif containing 59(TRIM59) on the biological function of human colorectal cancer HCT116 cells and its possible mechanism.
Methods:The recombinant TRIM59 interference plasmid(si-TRIM59) was constructed and transfected into colorectal cancer cell line HCT116 by liposome transfection. The transfection efficiency was verified by RT-qPCR and Western blot. The effects of TRIM59 gene silencing on the proliferation, colony-formation ability and cell cycle of colorectal cancer cells were detected by CCK-8 method, colony formation assays and flow cytometry respectively. Western blot was used to detect the expression level of CDK4 and cyclinD1.
Results:The expression levels ofTRIM59mRNA and protein in colorectal cancer cells were significantly higher than those in normal colorectal mucosa cells(P<0. 05). After knockdown of TRIM59expression, the proliferation activity and colony forming ability of HCT116 cells were significantly inhibited(P<0. 05), and the cell cycle was arrested in G0/G1 phase. At the same time, the expression levels of CDK4and CyclinD1 significantly decreased(P<0. 05).
Conclusion:TRIM59was highly expressed in colorectal cancer cells. Down regulation ofTRIM59expression can inhibit the proliferation and clone formation of colorectal cancer cells, suggesting thatTRIM59may become a new target for gene therapy of colorectal cancer.
- Full text:2025022015311426281TRIM59基因沉默对结直...癌细胞增殖和细胞周期的影响_高晓斌.pdf
