Grade Evaluation of Color Sorting Lonicerae Japonicae Flos Based on HPLC Fingerprint and Index Components Combined with Multivariate Statistics
10.13748/j.cnki.issn1007-7693.20223295
- VernacularTitle:基于HPLC指纹图谱与指标性成分结合多元统计学对色选金银花等级的评价研究
- Author:
HAO Peijun
1
;
ZHANG Linxiang
1
;
JIN Wanjun
2
;
NI Lin
2
;
QIU Guoyu
3
;
ZHAI Yusheng
4
;
LEI Chunming
4
;
SONG Pingshun
2
Author Information
1. Gansu University of Traditional Chinese Medicine, Lanzhou 730030, China
2. Gansu Provincial Institute of Pharmaceutical Inspection, National Key Laboratory for Quality Control of Chinese Medicinal Materials and Pieces, Gansu Provincial Engineering Laboratory for Testing Technology of Chinese and Tibetan Medicines, Lanzhou 730030, China
3. Gansu Pharmaceutical Group Science and Technology Innovation Research Institute Limited, Lanzhou 730030, China
4. Tongwei County Qingliang Yuan Honeysuckle Industry Poverty Alleviation Development Co., Ltd., Dingxi 743300, China
- Publication Type:Journal Article
- Keywords:
Lonicerae Japonicae Flos;
fingerprint;
cluster analysis;
quality evaluation;
grade standard
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(19):2694-2701
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the correlation evaluation and quality evaluation method of HPLC fingerprint grade of color sorting Lonicerae Japonicae Flos, and provide technical basis for the grade standard of color sorting Lonicerae Japonicae Flos. METHODS The chromatographic column was SVEA C18(250 mm×4.6 mm, 5 μm); mobile phase was acetonitrile(A)- 0.2% formic acid aqueous solution(B); gradient elution; injection volume was 10 μL; detection wave length was 245 nm; volume flow rate was 0.5 mL·min-1; column temperature was 38 ℃. The common peak determination and similarity evaluation of HPLC chromatogram data were carried out by using the Similarity Evaluation System of Traditional Chinese Medicine Chromatographic Fingerprints(Version 2012); the color sorting grade evaluation was carried out by CA, PCA and PLS-DA. The first part of Chinese Pharmacopoeia 2020 Edition was used to measure the quality control indicators, and the data were analyzed comprehensively. RESULTS A total of 28 common peaks were identified in the fingerprints, and 7 components were identified. The similarity of 24 batches of color sorting grade samples was 0.936-0.968. CA and PCA divided 28 batches of Lonicerae Japonicae Flos samples into 4 categories, which were basically consistent with the classification of color sorting, and PLS-DA achieved a discrimination result that was very consistent with the classification of color sorting. The color sorting grade was negatively correlated with the diameter, flowering rate, damage rate, and luteolin content of Lonicerae Japonicae Flos. The color sorting grade was positively correlated with chlorogenic acid, 3,5-di-O-caffeoyl quinic acid and 4,5-di-O-caffeoyl quinic acid. There was a clear correlation between the color sorting of Lonicerae Japonicae Flos and established fingerprint overall. There were differences in the quality of Lonicerae Japonicae Flos in the color sorting grade. Based on the sensory indicators of diameter, flowering rate, and damage rate, the content, diameter, flowering rate, and damage rate of luteolin showed a trend from high to low, ranging from third grade>second grade>first grade>special grade. The content of three phenolic acids showed a trend from high to low, ranging from special grade>first grade>second grade>third grade. Among the special grade, the content of three phenolic acids was the highest. CONCLUSION Combining the content of luteolin and phenolic acids as evaluation and control indicators for color selection grade is feasible and scientific, which can achieve intelligent color sorting grade production of Lonicerae Japonicae Flos grade.
