Screen the Volatile Oil β-Cyclodextrin Infused Process of the Classic Prescription Recipe Sanhua Decoction Based on Plackett-Burman Combined with Box-Behnken
10.13748/j.cnki.issn1007-7693.20223429
- VernacularTitle:基于Plackett-Burman结合Box-Behnken优选经典名方三化汤挥发油β-环糊精包合工艺
- Author:
DENG Yawei
1
;
LIU Mingsong
1
;
LI Xinnong
1
;
LI Chunhua
1
,
2
,
3
,
4
;
XIN Xiaodong
1
Author Information
1. College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, China
2. Hebei Higher Education Institute Applied Technology Research Center on TCM Formula Preparation, Shijiazhuang 050091, China
3. Hebei Technology Innovation Center of TCM Formula Preparations, Shijiazhuang 050091, China
4. Hebei Industrial Technology Institute for Traditional Chinese Medicine Preparation, Shijiazhuang 050000, China
- Publication Type:Journal Article
- Keywords:
classic prescription;
Sanhua decoction;
volatile oil;
saturated aqueous solution method;
headspace-GC-MS
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(19):2669-2676
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the cladding process of volatile oil saturated and aqueous solution method of classic prescription Sanhua decoction. METHODS β-Cyclodextrin was used as the wrapping material, through Plackett-Burman combined with Box-Behnken design tests, the optimal packaging process of Sanhua decoction’s total volatile oil was screened. The clathrate was verified by microscopy, thin layer chromatography, and ultraviolet spectrophotometry, and the change of volatile composition before and after the envelope was determined by headspace-gas chromatography-mass spectrometry. RESULTS The best coating process of the total volatile oil of the classic prescription Sanhua decoction was as follows: speed 2 000 r·min-1, wrapping 1 h, feed-liquid ratio 1∶10, baling temperature 30 ℃, material ratio 1∶9. The comprehensive scores of the three validation experiments were 0.981 0, 0.999 9 and 0.963 4, and the average value was 0.981 4, which was 2.87% different from the predicted value of the model. CONCLUSION The saturated aqueous solution method is simple and stable, and the volatile components are basically unchanged before and after the encapsulation, which provides a reference for the development of Sanhua decoction and the research on the volatile oil in the classic recipe.
