UPLC-QTOF/MS based investigation on metabolomics of cecal luminal contents in Balb/c mice infected with respiratory syncytial virus
10.19405/j.cnki.issn1000-1492.2022.05.006
- Author:
Hongya Gui
1
;
Shumei Wang
1
;
Xiaoyan Zhang
1
;
Xiaocheng Zhang
1
;
Shenghai Huang
1
;
Maozhang He
1
Author Information
1. Dept of Microbiology, Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
respiratory syncytial virus;
cecum;
UPLC⁃QTOF/MS;
metabolomics;
metabolic disorder
- From:
Acta Universitatis Medicinalis Anhui
2022;57(5):702-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Balb/c mice infected with respiratory syncytial virus(RSV) were used to investigate the metabolic changes in cecal luminal content.
Methods:A total of 13 female Balb/c mice were randomly divided into Case group(n=7) and control(Ctrl) group(n=6). Animals in Case group were infected with RSV by using intranasal method, while mice in Ctrl group were treated with DMEM medium. Mice were anesthetized with intraperitoneal administration of 10% chloral hydrate and the cecal luminal contents were harvested under sterile conditions. Metabolite concentrations were measured by UPLC-QTOF/MS system. Univariate and multivariate statistical analysis were used to identify differential metabolites between Case and Ctrl groups.
Results:The overall base peak chromatogram between Case and Ctrl groups had a clear disparity, and PCA and OPLS-DA analysis showed obviously discrepancy based overall metabolomic profile. L-serine, 2-ketobutyric acid, Oleic acid and Chenodeoxycholic acid glycine conjugate were enriched in Case group, whereas L-methionine, L-tyrosine and Nicotinic acid were depleted. Pathway analysis showed lysine degradation, Cysteine and methionine metabolism were enriched.
Conclusion:Lung injury induced by RSV infection may cause the endogenous metabolism disorder of cecal contents.
- Full text:2025021914585055317基于UPLC-QTOF-M...感染鼠盲肠内容物代谢组研究_桂红芽.pdf
