Nasal Administration of NMFGF1 Loaded Nanoparticles Improved Cognitive Dysfunction in Vascular Dementia Mice
10.13748/j.cnki.issn1007-7693.20231006
- VernacularTitle:鼻腔给药NMFGF1纳米粒改善血管性痴呆小鼠认知功能障碍
- Author:
ZHANG Ming
1
;
SUN Minyi
1
;
ZHU Ningwei
2
;
CHEN Mengjia
1
;
ZHAO Xichun
3
Author Information
1. Ningbo Yinzhou Second Hospital, Ningbo 315100, China
2. Zhejiang Pharmaceutical College, Ningbo 300052, China
3. Hefei BOE Hospital, Heifei 230000, China
- Publication Type:Journal Article
- Keywords:
nanoparticles;
nasal administration;
vascular dementia;
NMFGF1;
oxidative stress
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(20):2838-2845
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of nasal administration of non-mitogenic acid fibroblast growth factor(NMFGF1) loaded nanoparticles(NMFGF1-NPs) on the improvement of cognitive function in vascular dementia(VD) mice and its mechanism. METHODS Nanoparticles containing NMFGF1(NMFGF1-NPs) were prepared by water-in-water emulsion technique and characterized. The mice were divided into sham group, VD model group, blank-NPs group, NMFGF1 solution group and NMFGF1-NPs group after repeated cerebral ischemia-reperfusion to establish VD test model, and then given the corresponding form of drug intervention by nasal cavity. After drug intervention, Morris water maze was used to evaluate the learning and memory function of the animals in each group from the perspective of behavior. Meanwhile, the morphology, arrangement and apoptosis index(AI) of hippocampal neurons in each group were evaluated by pathological methods such as HE staining, FJB staining and Tunel apoptosis staining. In addition, ELISA and Western blotting were used to investigate the molecular mechanism of NMFGF1-NPs improving VD by nasal administration. RESULTS The morphology of NMFGF1-NPs was round. The encapsulation rate of NMFGF1-NPs respectively was (87.76±5.89)%. Morris water maze results showed that the behavioral indexes of mice in VD model group were significantly different from those in sham operation group(P<0.01). At the same time, the pathological results showed that the neurons in the CA1 region of the hippocampus in the VD model group were disordered, the cells morphology and structure were missing, and the AI was significantly increased compared with that in the sham operation group(P<0.01). Meanwhile, compared with the VD model group, the NMFGF1-NPs treatment group showed significant improvement in various behavioral indexes, and the hippocampal neuron cells were intact and orderly, and the AI index was significantly decreased(P<0.01). ELISA and Western blotting analysis showed that compared with that of VD model group and other intervention groups, the content of MDA in the brain of NMFGF1-NPs treatment group was significantly decreased. While the content of SOD, NO and the expressions of Nrf2, SOD-1 and GSTO1/2 was significantly increased (P<0.01). CONCLUSION Nasal administration of NMFGF1-NPs can play the role of antioxidant stress damage by activating Nrf2/ARE signal pathway, and ultimately improve the learning and cognitive function of VD mice.
