Effect of melatonin on embryo development after fertilization of in vitro matured oocytes in controlled ovarian hyperstimulation cycles
10.19405/j.cnki.issn1000-1492.2022.04.020
- Author:
Qi Zhu
1
,
2
;
Ding Ding
3
;
Kaijuan Wang
3
;
Ran Huo
4
;
Xuesen Zhang
4
;
Tingting Liu
5
;
Yunxia Cao
3
;
Zhiguo Zhang
1
,
2
,
6
,
6
,
6
Author Information
1. Dept of Biomedical Engineering，Anhui Medical University，Hefei 230032
2. Dept of Obstetrics and Gynecology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
3. Dept of Obstetrics and Gynecology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
4. State Key Laboratory of Reproductive Medicine，Nanjing Medical University，Nanjing 211166
5. Medical Record Information Office of Anhui Thoracic Hospital，Hefei 230022
6. Dept of Obstetrics and Gynecology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022Dept of Biomedical Engineering，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
melatonin;
embryo culture in vitro;
embryonic development;
aneuploidy
- From:
Acta Universitatis Medicinalis Anhui
2022;57(4):616-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of melatonin(MT) on the fertilization of in vitro matured oocytes in controlled ovarian hyperstimulation(COH) cycles and the development of subsequent embryos.
Methods:Imma-ture human oocytes from COH cycles were collected for in vitro maturation(IVM) culture and intracytoplasmic sperm injection( ICSI) insemination,and then the cleavage embryo and blastocyst culture medium supplemented with 0,10-11,10-9,10-7or 10-5mol/L MT were used in order to perform embryo culture in vitro,next the formed high-quality blastocysts were picked up and cryopreserved via vitrification,finally,array-CGH technology was used to detect aneuploidy of rewarmed high-quality blastocysts.
Results:The blastocyst rate of 10-9mol/L group was significantly higher than that of 10-11,10-7,10-5and 0 mol/L groups( P<0. 05,P<0. 01,P<0. 000 1,P<0. 01),respectively; the high-quality blastocyst rate of 10-9mol/L group was higher than that of other groups,but there were only significant differences compared with 10-5mol/L and 0 mol/L groups( P<0. 01,P<0. 05); the incidence of aneuploidy in MT group( 17. 6%) was lower than that in non-MT group( 33. 3%),but no significant difference was found.
Conclusion:The addition of MT to human embryo culture medium can promote the in vitro development of embryo,which is related to concentration,and 10-9mol/L is the optimal concentration.
- Full text:2025021815191747634褪黑素对控制性超排卵周期中...胞受精后所获胚胎发育的影响_朱琦.pdf
