Effects of embryonic inflammation on aging hippocampal SNAP-25 and cognitive function
10.19405/j.cnki.issn1000-1492.2022.04.018
- Author:
Qiyao Wei
1
;
Yongfang Wu
2
;
Yuxin Zhang
1
;
Yatao Wang
2
;
Liping Zeng
2
;
Guihai Chen
2
;
Lei Cao
1
Author Information
1. Dept of Neurology，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601
2. Dept of Neurology(Dept of Sleep Disorders) ，The Affiliated Chaohu Hospital of Anhui Medical University，Hefei 238000
- Publication Type:Journal Article
- Keywords:
aging;
inflammation;
learning and memory;
SNAP-25
- From:
Acta Universitatis Medicinalis Anhui
2022;57(4):604-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of embryonic inflammation on the hippocampal synaptosomal-associated protein 25(SNAP-25) level and cognitive function in middle-aged.
Methods:During gestational days 15-17, the CD-1 maternal mice received a daily intraperitoneal injection of lipopolysaccharides(LPS, 50 μg/kg) or the equal volume of normal saline, and the corresponding offspring were regarded as LPS group and CON group respectively. At the age of young(3-month-old) and middle-aged(15-month-old), the spatial learning and memory ability was assessed using Morris water maze(MWM), and the expression of hippocampal SNAP-25 protein was detected by immunohistochemical method and Western blot.
Results:Compared with the 3-month CON group, the 15-month CON group had longer swimming distance(P<0.01), lower swimming distance percentage(P<0.01) in the target quadrant, and higher hippocampal subregions(CA1, CA3, DG) SNAP-25 levels(P<0.01). The same results were obtained in 15-month LPS group compared with 15-month CON group in learning and memory phase(P<0.05), and higher hippocampal subregions(CA1, DG) SNAP-25 levels(P<0.01). Pearson correlation analysis indicated that the hippocampal CA1 and CA3 subregions SNAP-25 level was positively correlated with the swimming distance, but negatively correlated with the percentage of swimming distance in the target quadrant.
Conclusion:Embryonic inflammation can accelerate the impairment of spatial learning and memory and the increase of hippocampal CA1 and CA3 subregions SNAP-25 protein in middle-aged CD-1 mice, and there may be a correlation between them.
- Full text:2025021815050678198胚胎期炎症影响衰老海马SNAP-25含量及认知功能_韦琪瑶.pdf
