Cx43 regulates NLRP3 inflammasome and participates in α1-AR activation induced cardiac acute sympathetic stress
10.19405/j.cnki.issn1000-1492.2022.04.006
- Author:
Wenbo Wang
1
,
2
,
3
;
Yi Rong
1
,
2
,
4
;
Ling Chen
1
,
2
,
3
;
Xinzhi Li
1
,
2
,
4
;
Junqiang Si
1
,
2
,
4
;
Li Wang
1
,
2
,
3
;
Ketao Ma
1
,
2
,
4
Author Information
1. Ministry of Education Key Laboratory of Xinjiang Endemic and Ethnic Diseases，School of Medicine， Shihezi University，Shihezi 832000
2. NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases，Shihezi 832000
3. Dept of Cardiology，First Affiliated Hospital，School of Medicine， Shihezi University，Shihezi 832000
4. Dept of Physiology，School of Medicine，Shihezi University，Shihezi 832000
- Publication Type:Journal Article
- Keywords:
connexin 43;
NLRP3 inflammasome;
phenylephrine;
myocardial cells;
acute sympathetic stress
- From:
Acta Universitatis Medicinalis Anhui
2022;57(4):534-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of connexin 43 ( Cx43) in acute sympathetic stress induced by phe- nylephrine (PE) overactivation of α1-adrenergic receptor ( α1-AR) in cardiomyocytes．
Methods:Cardiomyocytes of H9C2 rats were randomly divided into control group，PE alone treatment group，Gap26 ( Cx43 specific inhibitor) intervention group and Gap26 alone treatment group．PE alone treatment group was treated with 50 μmol / L PE for 15 min．The Gap26 intervention group was pretreated with 0. 5 μmol / L Gap26 for 30 min，and then treated with 50 μmol / L PE for 15 min．The protein and mRNA expression levels of Cx43，NLRP3 inflammasome，interleukin-1 β (IL-1 β) ，Caspase-1，interleukin-18 (IL-18 ) were detected by Western blot and qRT-PCR. The expression and co-location of Cx43 in cardiomyocytes were observed by immunofluorescence assay，and the expression of inflamma- tory cytokines IL-1 β and IL-18 in cardiomyocytes was detected by ELISA.
Results:Compared with control group， the protein and mRNA levels of Cx43，NLRP3，Caspase-1 and IL-18 in PE group increased．Compared with PE a- lone treatment group，the protein and mRNA levels of Cx43，NLRP3，Caspase-1 and IL-18 decreased after Gap26 intervention，but they were still higher than those of control group． Similarly ，immunofluorescence showed that Cx43 protein expression increased in PE alone group，while Cx43 expression was down-regulated in Gap26 inter- vention group compared with PE alone group．ELISA results showed that the expression of IL-1 β and IL-18 was sig- nificantly up-regulated in PE alone group，but down-regulated in Gap26 intervention group．
Conclusion:Cx43 is involved in α1-AR activation induced cardiac acute sympathetic stress by regulating NLRP3 inflammasome.
- Full text:2025021808445472753Cx43调控NLRP3炎性...激活诱导的心脏急性交感应激_王文博.pdf
