Research on the extraction process of standard decoction of Longan seed based on antioxidant activity
- VernacularTitle:基于抗氧化活性的龙眼核标准汤剂提取工艺研究
- Author:
Jiali JIANG
1
,
2
,
3
;
Yijie WANG
1
,
2
,
3
;
Tanfang XIE
1
,
3
;
Zhiping WANG
1
,
2
Author Information
1. College of Pharmacy，Guangxi University of Chinese Medicine，Nanning 530200，China
2. Collaborative Innovation Center of Research on Functional Ingredients from Agricultural Residues，Nanning 530200，China
3. Key Laboratory of Generic Technology Research and Development of Traditional Chinese Medicine Preparations in Colleges and Universities of Guangxi，Nanning 530200，China
- Publication Type:Journal Article
- Keywords:
Longan seed;
standard decoction;
antioxidant activity;
orthogonal experiment;
extraction process
- From:
China Pharmacy
2025;36(3):330-335
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the extraction process of standard decoction of Longan seed. METHODS The amount of water added， extraction time and extraction times as the influencing factors， using the extract yield， gallic acid content， corilagin content， ellagic acid content， half clearance concentration （IC50） of 1，1-diphenyl-2-picryl-hydrazyl （DPPH） free radical and IC50 of 2，2′-azino-bis （3-ethylbenzothiazoline-6-sulfonic acid） ammonium salt （ABTS） free radical as the evaluation indexes， the analytic hierarchy process （AHP）， criteria importarue though inter-criteria correlation （CRITIC） and AHP-CRITIC method were used to calculate the weight values of each index； the extraction process of Longan seed standard decoction was optimized by single factor test combined with orthogonal experiment； validation test was also performed. RESULTS According to the AHP-CRITIC method， the weights of the above indicators were determined to be 9.224%， 11.784%， 19.320%， 11.206%， 20.597%， 27.869%， respectively. The best process of standard decoction of longan seed was to add 14 times water for the first extraction and extract for 30 minutes； and 12 times water for the second and third extractions， and extract for 20 minutes. Average comprehensive score of the 3 times validation experiments was 97.96 and the RSD was 0.97% （n＝3）. CONCLUSIONS The process is simple to operate， stable and feasible， which can provide a experimental basis for the further development and utilization of standard decoction of longan seed.
