Effects and mechanism of osthol on skin wound healing and angiogenesis in rats
- VernacularTitle:蛇床子素对大鼠皮肤创面愈合和血管生成的影响及机制
- Author:
Xiyan FEI
1
;
Dan WANG
1
;
Juan JIANG
1
;
Xinfang HE
1
;
Enjing ZHANG
2
;
Shuqi FEI
3
Author Information
1. Dept. of Obstetrics and Gynecology，Wuhan Third Hospital，Wuhan 430060，China
2. Dept. of Pharmacy，Wuhan Third Hospital，Wuhan 430060，China
3. Dept. of Pharmacy，Huangmei County Second People’s Hospital of Hubei Province，Hubei Huanggang 435502，China
- Publication Type:Journal Article
- Keywords:
osthole;
sonic hedgehog signaling pathway;
wound healing;
skin;
angiogenesis
- From:
China Pharmacy
2025;36(3):324-329
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of osthole （OST） on skin wound healing and angiogenesis in rats by regulating the sonic hedgehog （SHH） signaling pathway. METHODS Full-layer skin defect wound model rats were established and then randomly separated into Model group， OST low-dose， medium-dose and high-dose groups （OST-L group， OST-M group， OST-H group， 20， 30， 40 mg/kg OST）， high-dose OST+SHH inhibitor cyclopamide group （OST-H+cyclopamide group， 40 mg/kg OST+10 mg/kg cyclopamide）， with 12 rats in each group. Another 12 rats were selected as the control group. The wound healing of rats on 1， 7 and 14 days of administration was observed， and the wound healing rate of rats in each group was measured. The pathological changes and collagen deposition in rat wound tissue were observed； the levels of angiopoietin-1 （Ang-1） and basic fibroblast growth factor （bFGF） in wound tissue of rats were detected； the relative expressions of vascular endothelial growth factor A （VEGFA） and vascular endothelial growth factor receptor-2 （VEGFR-2） mRNA were also detected in wound tissue of rats； the protein expressions of VEGFA， VEGFR-2， SHH and glioma-associated oncogene homolog-1 （GLI1） were determined in wound tissue of rats. RESULTS Compared with Model group， the healing rate of skin wound， relative expression of collagen protein， the levels of Ang-1 and bFGF， the mRNA and protein expressions of VEGFA and VEGFR-2， and the protein expressions of SHH and GLI1 were all significantly increased in OST-M and OST-H groups （P＜0.05）. The wound tissue underwent significant re- epithelialization， with reduced inflammatory cell infiltration and granulation tissue edema， and an increase in the number of new blood vessels. SHH inhibitor cycloparamide weakened the promoting effects of OST on skin wound healing and angiogenesis in rats. CONCLUSIONS OST may promote skin wound healing and angiogenesis in rats by activating the SHH signaling pathway.
