Mechanism of Xinnao shutong capsule alleviating cerebral ischemia-reperfusion injury in rats by regulating ferroptosis
- VernacularTitle:心脑舒通胶囊抑制铁死亡减轻大鼠脑缺血再灌注损伤的机制
- Author:
Huani LI
1
,
2
;
Changhe LIU
1
,
2
;
Xiaoyan GUO
1
,
2
;
Xin ZHONG
3
;
Wei ZHANG
1
,
2
;
Wenjing GE
1
Author Information
1. Academy of Chinese Medicine，Henan Integrative Medicine Hospital，Zhengzhou 450004，China
2. Henan Engineering Research Center of Traditional Chinese Medicine Preparation，Zhengzhou 450004，China
3. Dept. of Pharmacy，Zhengzhou Cigarette Factory Rehabilitation Hospital，Zhengzhou 450047，China
- Publication Type:Journal Article
- Keywords:
Xinnao shutong capsule;
cerebral ischemia-
- From:
China Pharmacy
2025;36(3):306-311
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the mechanism of Xinnao shutong capsule alleviating cerebral ischemia reperfusion injury （CIRI） in rats by regulating the ferroptosis pathway. METHODS SD rats were randomly divided into sham operation group， model group， Xinnao shutong low-dose， high-dose group （220， 440 mg/kg）， Ginkgo biloba leaves extract group （positive control， 150 mg/kg）. Each group of rats was orally administered with the corresponding medication/normal saline for 7 consecutive days. Transient occlusion of the middle cerebral artery was adopted to induce the CIRI model； the samples were taken 24 h after the operation； the cerebral infarction area of rats was detected， and the cerebral infarction rate was calculated. The pathological changes of brain tissues were observed， and the levels of lipid peroxide （LPO）， malondialdehyde （MDA） and glutathione （GSH） in cerebral tissue were detected； mRNA and protein expressions of nuclear factor-erythroid 2-related factor 2 （Nrf2）， heme oxygenase 1（HO-1） and glutathione peroxidase 4 （GPX4） were all detected in cerebral tissue of rats. RESULTS Compared with model group， the cerebral infarction rate， the content of total iron in cerebral tissue and serum level of LPO （except for Ginkgo biloba leaves extract group and Xinnao shutong low-dose group） were all decreased significantly in G. biloba leaves extract group and Xinnao shutong groups （P＜0.05 or P＜0.01）； the serum level of GSH， the protein and mRNA expressions of Nrf2， HO-1 and GPX4 were all increased significantly （P＜0.05 or P＜0.01）. The pathological damage to brain tissue was reduced， the number of nerve cells increased， the edema was alleviated， and the nuclear membrane was flattened. CONCLUSIONS Xinnao shutong capsule can inhibit ferroptosis and reduce CIRI， the mechanism of which may be associated with the activation of the Nrf2/HO-1/GPX4 signaling pathway.
