Application of serological methods combined with flow cytometry in the detection of immune hemolytic transfusion reaction
10.13303/j.cjbt.issn.1004-549x.2025.01.020
- VernacularTitle:血清学联合流式细胞技术在免疫性溶血性输血反应相关检测中的应用
- Author:
Lei LIU
1
,
2
;
Qunjuan ZENG
3
;
Guoqin GONG
1
;
Dong XIANG
1
;
Zhongying WANG
1
Author Information
1. Shanghai Blood Center, Shanghai 200051, China
2. Jilin City Hospital of Chemical Industry, Jilin 132000, China
3. Dazhou Central Hospital, Dazhou 635000, China
- Publication Type:Journal Article
- Keywords:
flow cytometry;
anti-C, e;
anti-Jka;
capillary centrifugation technology;
hemolytic transfusion reaction
- From:
Chinese Journal of Blood Transfusion
2025;38(1):116-121
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To explore the effects of different methods on antibody detection through investigating the causes of cross-matching incompatible in a patient with gastric malignant tumor, and to establish flow cytometry protocol for confirming hemolytic transfusion reaction (HTR). [Methods] Antibodies in the patient's serum were identified by red blood cells (RBCs) blood grouping, antibody screening and identification, acid elution test and PEG enhancement test. To confirm HTR, patient RBCs, proximal and distal ends RBCs, separated by capillary centrifugation, were tested by direct antiglobulin test (DAT) and Jka antigen single label and double label flow cytometry. [Results] Routine serological technology revealed the presence of anti-C, e (titer:2) and anti-Jka (titer >1) in the patient’s serum. After separation using capillary centrifugation technology, both the proximal and distal DAT and Jka antigen tests were negative. Both DAT and Jka antigen positive red blood cells (0.21%, 6/6 327) were found in the patient's blood samples by flow cytometry. After separation of blood samples by capillary centrifugation, there were significantly more DAT and Jka antigen double-positive RBCs in the distal end (0.43%, 33/7 707) than in the proximal end (0.09%, 15/7 225). Two blood samples were screened from over 100 donor blood samples that are compatible with the patient's cross-matching, and the transfusion effect was favorable. [Conclusion] Serological methods combined with flow cytometry could improve the sensitivity of antibody detection, provide a more accurate basis for the diagnosis of HTRs, and guarantee the safety of blood transfusion.
