Molecular biological research and molecular homologous modeling of Bw.03 subgroup

Li Wang; Yongkui Kong; Huifang Jin; Xin Liu; Ying Xie; Xue Liu; Yanli Chang; Yafang Wang; Shumiao Yang; Di Zhu; Qiankun Yang

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Molecular biological research and molecular homologous modeling of Bw.03 subgroup

VernacularTitle:Bw.03的分子生物学研究及分子同源建模
Author: Li WANG ¹ ; Yongkui KONG ¹ ; Huifang JIN ¹ ; Xin LIU ¹ ; Ying XIE ¹ ; Xue LIU ¹ ; Yanli CHANG ¹ ; Yafang WANG ¹ ; Shumiao YANG ¹ ; Di ZHU ¹ ; Qiankun YANG ¹
Author Information

1. Department of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Publication Type:Journal Article
Keywords: Bw blood group; molecular biology; homologous modeling; intermolecular force analysis
From: Chinese Journal of Blood Transfusion 2025;38(1):112-115
CountryChina
Language:Chinese
Abstract: [Objective] To study the molecular biological mechanism for a case of ABO blood group B subtype, and perform three-dimensional modeling of the mutant enzyme. [Methods] The ABO phenotype was identified by the tube method and microcolumn gel method; the ABO gene of the proband was detected by sequence-specific primer polymerase chain reaction (PCR-SSP), and the exon 6 and 7 of the ABO gene were sequenced and analyzed. Homologous modeling of Bw.03 glycosyltransferase (GT) was carried out by Modeller and analyzed by PyMOL2.5.0 software. [Results] The weakening B antigen was detected in the proband sample by forward typing, and anti-B antibody was detected by reverse typing. PCR-SSP detection showed B, O gene, and the sequencing results showed c.721 C>T mutation in exon 7 of the B gene, resulting in p. Arg 241 Trp. Compared with the wild type, the structure of Bw.03GT was partially changed, and the intermolecular force analysis showed that the original three hydrogen bonds at 241 position disappeared. [Conclusion] Blood group molecular biology examination is helpful for the accurate identification of ambiguous blood group. Homologous modeling more intuitively shows the key site for the weakening of Bw.03 GT activity. The intermolecular force analysis can explain the root cause of enzyme activity weakening.