Genetic analysis of weak expression of ABO blood group antigens in neonates
10.13303/j.cjbt.issn.1004-549x.2025.01.014
- VernacularTitle:新生儿ABO血型抗原弱表达的基因分析
- Author:
Jiali YANG
1
;
Ding ZHAO
1
;
Wei LI
1
;
Xiaopan ZHANG
1
;
Zhihao LI
1
;
Dongdong TIAN
1
Author Information
1. Department of Blood Transfusion, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450018, China
- Publication Type:Journal Article
- Keywords:
neonate;
ABO blood group;
weak expression of antigen;
ABO subtype;
gene sequencing
- From:
Chinese Journal of Blood Transfusion
2025;38(1):85-90
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To perform genetic analysis on samples with weak agglutination and mixed agglutination of ABO blood group antigens in neonates, and to investigate the molecular biological characteristics of ABO subtypes in neonates. [Methods] Serological identification of ABO blood group was performed by tube method and microcolumn gel method. The ABO exons 2-7 were amplified by PCR, and the amplified products were sequenced by Sanger sequencing method to determine the genotype. [Results] Among the ABO blood group serological results of 14 neonates, 8 cases showed weakened A antigen, and 6 cases showed weakened B antigen. Seven samples were identified with ABO subtype alleles, with genotypes as A102/B101+c.538C>T, Aw26/B102, A205/O02, A205/B101(2 cases), Aw26/O02, B(A)06/O01, B101/O01(3 cases), A102/O01(2 cases), A102/B101 and B101/O02. Additionally, three other family members were also found to carry B(A)06 allele in a pedigree investigation. [Conclusion] For samples showing weakened antigens in ABO blood type identification of neonates, it is necessary to consider the possibility of ABO subtype in addition to age factors, and genetic testing can be used to prevent missed detection of ABO subtypes in neonates.
