Analysis of Animal Models of Allergic Asthma Based on Data Mining
10.13422/j.cnki.syfjx.20241712
- VernacularTitle:基于数据挖掘的过敏性哮喘动物模型应用分析
- Author:
Han WU
1
;
Zhixiang HU
1
;
Meiqi JI
1
;
Hao YIN
1
;
Yu'e LYU
1
;
Chuntao ZHAI
1
Author Information
1. Shanxi University of Chinese Medicine， Jinzhong 030600， China
- Publication Type:Journal Article
- Keywords:
allergic asthma;
data mining;
animal model;
application analysis;
test indicators
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(5):211-217
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo provide a basis for the establishment of an ideal animal model of allergic asthma by statistically analyzing the modeling characteristics and the selection of indicators of the available models. MethodsWe retrieved the relevant articles from China National Knowledge Infrastructure（CNKI）， VIP， Wanfang Data， SinoMed， and PubMed with "allergic asthma" as the keyword and the time interval from January 2019 to January 2024. Through integrating the literature and extracting data， we used Excel 2021 to create a personal database and sorted out the animal strains， genders， allergenic substances， modeling routes， and test indicators and methods. Excel 2021， Cytoscape 3.10.2， and SPSS Modeler 18.0 were then used to analyze the relevant characteristics of the animal models. ResultsA total of 418 articles were included in the database， and the comparative analysis showed that the most frequently used animal strain for modeling was BALB/c mice， and female animals were mostly used. The main modeling method was sensitization by intraperitoneal injection of ovalbumin （OVA）， which was combined with intranasal inhalation. The test indicators mainly included appearance signs， cellular analysis， lung histopathology， lung function indicators， and protein and gene expression in the lung. The test methods mainly involved pathological staining， enzyme-linked immunosorbent assay， immunohistochemistry， immunofluorescence， Western blot， and polymerase chain reaction（PCR） assays. ConclusionThere is no recognized modeling method or evaluation standard for the animal models of allergic asthma. Based on the results of data analysis， the OVA-induced allergic asthma model in BALB/c mice is recommended. The main criteria for evaluating the success of modeling are the general behavioral changes， the morphological changes of the airway and inflammatory cell infiltration in the lung tissue， the changes of pro-inflammatory and anti-inflammatory cytokines in the serum， and the alterations of inflammatory cells in the bronchoalveolar lavage fluid.
