Material Basis and Its Distribution in vivo of Qili Qiangxin Capsules Analyzed by UPLC-Q-Orbitrap-MS
10.13422/j.cnki.syfjx.20250263
- VernacularTitle:基于UPLC-Q-Orbitrap-MS分析芪苈强心胶囊的物质基础及其体内分布
- Author:
Jianwei ZHANG
1
;
Jiekai HUA
1
;
Rongsheng LI
1
;
Qin WANG
1
;
Xinnan CHANG
1
;
Wei LIU
1
;
Jie SHEN
1
Author Information
1. Third Grade Laboratory of Traditional Chinese Medicine（TCM） Preparations，National Administration of TCM，Shuguang Hospital Affiliated to Shanghai University of TCM，Shanghai 201203，China
- Publication Type:Journal Article
- Keywords:
Qili Qiangxin capsules;
chemical composition analysis;
component attribution;
ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Orbitrap-MS）;
tissue distribution;
material basis;
heart failure
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(5):185-193
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveBased on ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Orbitrap-MS）， the chemical constituents of Qili Qiangxin capsules was identified， and their distribution in vivo was analyzed. MethodsUPLC-Q-Orbitrap-MS was used to detect the sample solution of Qili Qiangxin capsules， as well as the serum， brain， heart， lung， spleen， liver and kidney tissues of mice after oral administration. Using the Thermo Xcalibur 2.2 software， the compound information database was constructed， and the molecular formulas of compounds corresponding to the quasi-molecular ions were fitted. Based on the information of retention time， accurate relative molecular mass and fragments， the compounds and their distribution in vivo were analyzed by comparing with the data of reference substances and literature. ResultsA total of 233 compounds， including 70 terpenoids， 60 flavonoids， 23 organic acids， 17 alkaloids， 20 steroids， 7 coumarins and 36 others， were identified or predicted from Qili Qiangxin capsules， 73 of which were identified matching with standard substances. Tissue distribution results showed that 71， 17， 38， 33， 32， 58 and 43 migrating components were detected in blood， brain， heart， lung， spleen， liver and kidney， respectively. Thirty-seven components were absorbed into the blood and heart， including quinic acid， benzoylaconitine benzoylmesaconine and so on. Fourteen components were absorbed into the blood and six tissues， including calycosin， methylnissolin， formononetin， alisol B， alisol A and so on. ConclusionThis study comprehensively analyzes the chemical components of Qili Qiangxin capsules and their distribution in vivo. Among them， astragaloside Ⅳ， salvianolic acid B， ginsenoside Rb1， ginsenoside Rb3， ginsenoside Rd， ginsenoside Rg3， calycosin-7-glucoside， and sinapine may be the important components for the treatment of heart failure， which can provide useful reference for its quality control and research on pharmacodynamic material basis.
