Correlation between human leukocyte antigen HLA-A gene polymorphism and HBV carrier

Li SHUAI; Shuai YANG; Junjie YE; Jingcheng YANG; Xiaoye FU; Bin XU; Hongwei LIU; Lü ZHOU; Xilin KANG; Jing GAO

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Correlation between human leukocyte antigen HLA-A gene polymorphism and HBV carrier

VernacularTitle:人类白细胞抗原HLA-A基因多态性与HBV携带的相关性研究
Author: Li SHUAI ¹ ; Shuai YANG ² ; Junjie YE ³ ; Jingcheng YANG ⁴ ; Xiaoye FU ¹ ; Bin XU ⁵ ; Hongwei LIU ¹ ; Lü ZHOU ¹ ; Xilin KANG ¹ ; Jing GAO ¹
Author Information

1. Yan’an Hospital Affiliated to Kunming Medical University, Kunming 650000
2. Yunnan Agricultural Vocational and Technical College, Kunming 650031
3. Yunnan Institute of Population and Family Planning Science and Technology, Kunming 650021
4. Yunnan Open University, Kunming 650504
5. Kunming Children’s Hospital, Kunming 650100, China
Publication Type:Journal Article
Keywords: human leukocyte antigen (HLA); genetic polymorphism; HLA-A2; hepatitis B virus (HBV)
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(6):1037-1040
CountryChina
Language:Chinese
Abstract: [Objective] To investigate the correlation between human leukocyte antigen (HLA) gene polymorphism and hepatitis B virus (HBV) infection. [Methods] Venous blood samples were collected from 501 healthy individuals undergoing physical examinations at Yan’an Hospital in Kunming, Yunnan Province. Enzyme linked immunosorbent assay (ELISA) was used to detect HBV halves. Based on the results of HBV half detection, the patients were divided into three groups: HBV carrier group, previous infection group, and healthy control group. The HLA-A antigen genotype was detected using polymerase chain reaction with sequence specific primers (PCR-SSP) genotyping technology, and the distribution frequency of HLA-A gene polymorphism was compared between HBV carrier group and healthy control group, as well as between previous infection group and healthy control group. SPSS17.0 software was used for data statistical analysis. [Results] In the healthy control group, the HLA-A2 positivity rate was 47.49%, and the allele frequency was 31.29%.The overall frequency of gene distribution in the healthy control group was consistent with the HLA-A allele table commonly and confirmed in China published by the Chinese Bone Marrow Bank. The HLA-A2 positivity rate and allele frequency in the HBV carrier group were 63.04% and 42.23%, respectively; The difference in HLA-A2 positivity rate and allele frequency among carriers was statistically significant (P<0.05). the HLA-A2 positivity rate and allele frequency in the HBV previous infection group were 56.14% and 35.97%, respectively, which did not significantly differ from those in the healthy control group (P>0.05). [Conclusion] HLA-A2 gene may be a susceptibility gene for chronic hepatitis B HBV carriers.