Effects of Huashi Runzao Prescription on apoptosis and function of the submandibular gland in Sj?gren's syndrome model mice by regulating Hippo-YAP/TAZ expression
10.3969/j.issn.1006-2157.2024.11.008
- VernacularTitle:化湿润燥方调控Hippo-YAP/TAZ表达对干燥综合征小鼠颌下腺细胞凋亡和功能的影响
- Author:
Ziwei HUANG
1
,
2
;
Qingwen TAO
;
Jiahe LIAO
;
Xinbo YU
;
Qian HE
;
Bojie TANG
;
Jing LUO
Author Information
1. 北京中医药大学研究生院 北京 100029
2. 中日友好医院中医风湿病科免疫炎性疾病北京市重点实验室
- Keywords:
Huashi Runzao Prescription;
Sj?gren's syndrome;
apoptosis;
submandibular gland;
Hippo signaling pathway;
aquaporin 5;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2024;47(11):1527-1540
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of modulating the Hippo-YAP/TAZ pathway using Huashi Runzao Prescription(HRP)on the apoptosis and salivary secretion function of submandibular gland cells in a naive non-obese diabetic(NOD/Ltj)mouse model of Sj?gren's syndrome(SS).Methods Eight-week-old female BALB/c mice were selected as the blank group.Eight-week-old female NOD/Ltj were randomly divided into the model,HRP-L,HRP-M,HRP-H,and hydroxychloroquine sulphate(HCQ)groups.After eight weeks of drug administration,the water consumption and salivary flow rate of each group were recorded.The histopathological damage of the submandibular gland in each group was determined using hematoxylin and eosin staining,the apoptosis rate of the submandibular gland was determined using TUNEL staining,and AQP5,Bax,Bcl-2,beclin-1,YAP,p-YAP,and TAZ expressions in submandibular gland tissues were determined using immunohistochemistry and Western blotting.Results Compared with the blank group,the Hippo-YAP/TAZ pathway was inhibited in the submandibular gland tissues of the model group,lymphocyte infiltration increased,water consumption increased,salivary flow rate decreased,AQP5 expression decreased,and submandibular gland cells apoptosis rate increased(P<0.05).Compared with all other administered groups,in the HRP-M group,the Hippo-YAZ/TAZ pathway was significantly activated,lymphocyte infiltration in submandibular gland tissues was reduced,water consumption was reduced,the salivary flow rate and AQP5 expression were increased,and the apoptosis rate of submandibular gland cells was reduced(P<0.05).Conclusion HRP reduced the pathological damage and apoptosis of submandibular gland cells in SS model mice.It improved the overall secretory function of submandibular gland tissues,which may be related to Hippo pathway activation and downregulating YAP/TAZ expression.
