Study on the pharmacological effects and mechanism of Gegen-Zhimu herb pair in preventing and treating Alzheimer's disease by UHPLC-Q/TOF-MS metabolomics strategy
10.12206/j.issn.2097-2024.202409035
- VernacularTitle:基于UHPLC-Q/TOF-MS代谢组学策略的葛根-知母药对防治阿尔茨海默病的药效与作用机制研究
- Author:
Liang CHAO
1
,
2
;
Hui WANG
3
;
Shuqi SHEN
3
;
Piaoxue YOU
4
;
Kaihong JI
5
;
Zhanying HONG
3
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China
2. Department of Pharmacy, Jiangsu Provincial Corps Hospital, Chinese People’s Armed Police Force.
3. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
4. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
5. Department of Gastroenterology, First Affiliated Hospital of Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
Alzheimer's disease;
metabolomics;
Gegen-Zhimu;
herb pair;
mechanism
- From:
Journal of Pharmaceutical Practice and Service
2025;43(1):30-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficacy of Puerariae lobatae radix （PLR） and Anemarrhenae Rhizoma （AR） in preventing and treating Alzheimer’s disease （AD） and explore its potential mechanism of action by LC-MS serum metabolomics strategy. Methods The AD rat model was established by administering aluminum chloride （AlCl3） and D-galactose （D-gal） for 20 weeks. The traditional Chinese medicine intervention group was given the PLR, AR, and PLR-AR extracts for 8 weeks by gavage. The model effect and efficacy were evaluated by Morris water maze test and biochemical indicators including SOD, NO, and MDA; Metabolomics research based on the UHPLC-Q/TOF-MS method was conducted, and relevant metabolic pathways were analyzed through the MetaboAnalyst online website. Results The learning and memory abilities of AD model rats were significantly decreased compared with the control group, and the levels of oxidative stress and lipid peroxides were significantly increased （P<0.05）, while the SOD content was decreased considerably （P<0.01）. The learning and memory abilities of AD model rats were improved, oxidative stress and lipid peroxidation levels were reversed, and serum SOD content was increased significantly after the intervention of PLR-AR, with better effects than single drugs. Through metabolomics, 70 differential metabolites were identified between the AD model group and the control group, mainly involving 10 pathways, including phenylalanine, tyrosine, and tryptophan biosynthesis, phenylalanine metabolism, and unsaturated fatty acid biosynthesis, et.al. The intervention of PLR-AR could adjust 47 metabolites, with 20 metabolites showing significant differences （P<0.05）. The significantly adjusted metabolites involve 6 pathways, including phenylalanine, tyrosine, and tryptophan biosynthesis, et al. Conclusion The combination of PLR and AR could significantly improve the learning and memory abilities of AD rat models. The mechanism may be related to the improvement of oxidative stress and lipid peroxidation levels, the increase of serum SOD content, and the regulation of phenylalanine, tyrosine, and tryptophan biosynthesis pathways.
