Establishment and analysis of chronic rejection model of mouse heart transplantation
10.12464/j.issn.1674-7445.2024215
- VernacularTitle:小鼠心脏移植慢性排斥反应模型的建立和分析
- Author:
Wei ZHANG
1
;
Qingrong ZHANG
1
;
Maolin MA
1
;
Qianghua LENG
1
;
Fei HAN
1
Author Information
1. Department of Kidney Transplantation, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
- Publication Type:OriginalArticle
- Keywords:
Heart transplantation;
Chronic rejection;
Animal model;
Mouse;
Cytotoxic T lymphocyte-associated antigen 4-immunoglobulin;
Donor specific antibody;
Immune cell;
Inflammatory cell infiltration
- From:
Organ Transplantation
2025;16(1):99-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a chronic rejection (CR) model of mouse heart transplantation and analyze its characteristics. Methods Allogeneic BALB/c and C57BL/6 mice were used as donor and recipient for heart transplantation, and intraperitoneal injection of cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (CTLA4-Ig) was given 1 and 2 days after surgery. Graft survival time, donor specific antibody (DSA) level, graft pathology and inflammatory cell infiltration were observed. Results In allogeneic transplantation model, graft survival time was prolonged after CTLA4-Ig treatment [(28.2±4.1) d vs. (7.0±0.7) d, P < 0.01]. The level of serum DSA-IgG increased at 2, 3 and 4 weeks after surgery, while the level of DSA-IgM remained unchanged. Myocardial cell injury, inflammatory cell infiltration, interstitial fibrosis and C4d deposition in capillaries were aggravated 3 weeks after operation and worsened 4 weeks after operation. The infiltrated immune cells were mainly macrophages, T cells and plasma cells. Conclusions Mouse allogeneic heart transplantation combined with CTLA4-Ig successfully establishes a CR model, which provides a basis for subsequent studies on the pathogenesis and intervention of CR.
