Serum metabolomics study in patients with occupational chronic lead poisoning
10.20001/j.issn.2095-2619.20241003
- VernacularTitle:职业性慢性铅中毒患者血清代谢组学研究
- Author:
Bingchen LIU
1
;
Jin XU
;
Yao SU
;
Wei WANG
;
Hong QIN
;
Na SUN
;
Chunping LI
Author Information
1. The Affiliated Wuxi Center for Disease Control and Prevention of Nanjing Medical University,Wuxi Center for Disease Control and Prevention, Wuxi, Jiangsu 214023, China
- Publication Type:Journal Article
- Keywords:
Lead;
Lead poisoning;
Chronic poisoning;
Serum;
Metabolomics;
Metabolite;
Metabolic pathway
- From:
China Occupational Medicine
2024;51(5):496-504
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of serum metabolites in patients with occupational chronic lead poisoning using non-targeted metabolomics, and to screen differential metabolic pathways. Methods A total of 14 patients with occupational chronic lead poisoning were selected as the poisoning group, and 14 healthy people without occupational hazard exposure history were selected as the control group using the judgment sampling method. Serum of the individuals from the two groups was collected. Non-targeted metabolomics technology based on ultra high performance liquid chromatography-tandem mass spectrometry was used to detect serum metabolite levels in the two groups. Differential metabolites (DMs) were screened by the principal component analysis, partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis, and related metabolic pathways were explored. Results The blood lead level in the poisoning group was higher than that in the control group (median: 359.59 vs 5.04 μg/L, P<0.01). There were significant differences in serum metabolites between the poisoning group and control group. After the combination of results from the positive and negative ion patterns, a total of 89 DMs were screened in serum of patients in the poisoning group, including 50 upregulated and 39 downregulated metabolites compared with the control group. The serum DMs of poisoning group were mainly enriched in arginine biosynthesis, ABC transporter, purine metabolism, choline metabolism in malignant tumor, glycerophospholipid metabolism and ether lipid metabolism compared with the control group (all P<0.05). Conclusion Abnormal changes of serum metabolic profile occurred in patients with occupational chronic lead poisoning. The metabolic pathways such as arginine biosynthesis, ABC transporter, purine metabolism, choline metabolism, glycerophospholipid metabolism and ether lipid metabolism may be involved in the occurrence and development of lead poisoning.
