Effects and mechanism of persimmon leaf extract on IEC-6 cell ferroptosis induced by H2O2
- VernacularTitle:柿叶提取物对H2O2诱导IEC-6细胞铁死亡的影响及机制研究
- Author:
Xuexia ZHANG
1
;
Min ZHOU
2
;
Hongyan ZHOU
3
;
Lifei WANG
4
;
Huani LI
1
;
Changhe LIU
1
;
Hongde XU
2
;
Mingli ZHANG
5
Author Information
1. Institute of Chinese Medicine，Henan Integrative Medicine Hospital，Zhengzhou 450004，China
2. School of Pharmacy，Zhengzhou University，Zhengzhou 450001，China
3. Dept. of Clinical Laboratory，Henan Integrative Medicine Hospital，Zhengzhou 450004，China
4. Dept. of Nephropathy，Second Affiliated Hospital of Zhengzhou University，Zhengzhou 450014，China
5. Dept. of Pulmonary，Henan Integrative Medicine Hospital，Zhengzhou 450004，China
- Publication Type:Journal Article
- Keywords:
persimmon leaf extract;
ulcerative colitis;
ferroptosis;
Nrf2/HO-1 signaling pathway
- From:
China Pharmacy
2025;36(1):64-70
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects and potential mechanism of persimmon leaf （PL） extract against ferroptosis induced by H2O2 in IEC-6 cells. METHODS Using IEC-6 cells as object， the effects of ferroptosis inhibitor ferrostatin-1 on IEC-6 cell viability induced by H2O2 were investigated； IEC-6 cells were divided into control group， H2O2 group， H2O2+PL 25 μg/mL group and H2O2+PL 50 μg/mL group. The levels of oxidant stress indexes [content of malondialdehyde （MDA）， activity of superoxide dismutase （SOD）， and levels of reactive oxygen species （ROS）]， mitochondrial membrane potential （MMP） as well as mRNA and protein expressions of nuclear factor-erythroid-2 related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， NADPH/quinone oxidoreductase-1 （NQO-1）， cystine/glutamate anti-porter （xCT）， glutathione peroxidase 4 （GPX4） and ferritin heavy chain （FTH） were detected. RESULTS Ferroptosis inhibitor ferrostatin-1 could significantly increase the survival rate of H2O2-induced cells （P＜ 0.01）. Compared with the control group， MDA content， ROS level， mRNA expressions of Nrf2 and NQO-1 as well as protein expressions of Nrf2 and HO-1 were increased or up-regulated significantly， while SOD activity， MMP， mRNA expressions of xCT， GPX4 and FTH as well as protein expressions of GPX4 and FTH were decreased or down-regulated significantly （P＜0.01 or P＜0.05）. Compared with the H2O2 group， oxidative stress Δ indexes of H2O2+PL 25， 50 μg/mL groups were reversed to different extents， MMP level was increased significantly， as well as mRNA and protein expressions of Nrf2， HO-1， NQO-1，xCT， GPX4 and FTH were up-regulated to different extents；there were statistical significances in some indexes between groups （P＜0.01 or P＜0.05）. CONCLUSIONS PL extract can alleviate mitochondrial membrane damage and abnormal accumulation of ROS caused by H2O2， which may be related to the inhibition of ferroptosis by activating the Nrf2/HO-1 signaling pathway.
