Application of molecular biology techniques in the diagnosis and treatment of tuberculosis
- Author:
GONG Wenping
;
CHEN Wen
- Publication Type:Review
- Keywords:
tuberculosis;
molecular biology;
loop-mediated isothermal amplification;
line probe assay;
GeneXpert;
whole genome sequencing
- From:
China Tropical Medicine
2024;24(1):12-
- CountryChina
- Language:Chinese
-
Abstract:
Tuberculosis (TB) is one of the most prevalent infectious diseases worldwide, causing a significant number of deaths. Traditional diagnostic methods have limitations, while the application of molecular biology techniques has opened new avenues for TB diagnosis and treatment. This article focuses on the introduction and discussion of the application status of four molecular biological methods, including Loop-Mediated Isothermal Amplification (LAMP) technology, Line Probe Assay (LPA) technology, GeneXpert, and Whole Genome Sequencing (WGS) technology. These novel techniques offer notable advantages over traditional TB diagnostic methods, such as high sensitivity and specificity for identifying Mycobacterium tuberculosis and detecting drug resistance. Furthermore, these technologies facilitate automated and rapid TB diagnosis and drug resistance testing, providing insights into the species and drug resistance information of M. tuberculosis, thereby supporting personalized treatment. In conclusion, the application of molecular biological methods in TB diagnosis and treatment has shown tremendous potential for improving diagnostic accuracy and personalized therapeutic efficacy, thus holding paramount importance for the control and prevention of TB.
- Full text:2025010910120875913.Application of molecular biology techniques in the diagnosis and treatment of tuberculosis.pdf
