Rapid Identification of the Chemical Components of Mongolian Medicine Sanzi Powder by HPLC-Q-Exactive-MS/MS
DOI:10.13748/j.cnki.issn1007-7693.20210426
- VernacularTitle:HPLC-Q-Exactive-MS/MS快速鉴定蒙药三子散化学成分
- Author:
XIA Huimin
1
;
ZHANG Huiwen
1
;
LIU Hong
1
;
LIANG Yue
2
,
3
;
WANG Huanyun
1
Author Information
1. School of Pharmacy, Inner Mongolian Medical University, Hohhot 010110, China
2. People'
3. s Hospital of Inner Mongolia Autonomous Region, Hohhot 010017, China
- Publication Type:Journal Article
- Keywords:
Sanzi powder / HPLC-Q-exactive-MS/MS / chemical constituents
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(21):3005-3014
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: OBJECTIVE To quickly qualitatively analyze the chemical components in Sanzi powder compound prescription by HPLC-Q-Exactive-MS/MS. METHODS SHIMADZU GIST C18 column(4.6 mm×150 mm, 5 μm) was used, using 0.1% formic acid water-methanol as mobile phase with gradient elution, the flow rate was 0.5 mL·min-1, the column temperature was 30 ℃. Under positive and negative ion modes, the primary and secondary mass spectrometry information of Sanzi powder was scanned. Qualitative attribution of each component in Sanzi powder was carried out based on the mass spectrometry information, molecular formula, and retention time of molecular ion peaks and fragment ions analyzed using total ion flow diagrams, combined with the molecular formula and structural formula searched in the Chemspider database and references. RESULTS Based on the analysis of the mass spectrometry cleavage rules and references of various components, 95 possible chemical components were preliminarily inferred, including 39 phenolic acids, 20 tannins, 9 organic acid esters, 5 monoterpenoids, 12 iridoids, 8 triterpenes and 2 flavonoids. Among them, 57 components were from Chebulae Fructus, 30 were from Gardeniae Fructus, 10 were from Toosendan Fructus, and among these compounds, rutin from Chebulae Fructus, Gardeniae Fructus and Toosendan Fructus. CONCLUSION The HPLC-Q-Exactive-MS/MS detection method has good separation and high sensitivity, and can quickly and efficiently infer various components in Sanzi powder. It establishes a fast and efficient analytical method for identifying the chemical components in Sanzi powder.
