Discrimination of Quality Markers of Feire Zhike Granule Based on Five Principles Coupled with Cobweb Model
DOI;10.13748/j.cnki.issn1007-7693.20224179
- VernacularTitle:基于“五原则”结合蛛网模型辨析肺热止咳颗粒质量标志物
- Author:
CHEN Jia
1
;
LI Wei
1
;
LIU Zheng
1
;
ZHOU Wenxu
1
;
LIU Xiang
1
;
XING Qichang
1
Author Information
1. Xiangtan Central Hospital, Xiangtan 411100, China
- Publication Type:Journal Article
- Keywords:
Feire Zhike granules / Q-Marker / five principles / cobweb model / network pharmacology
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(21):2990-2997
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:OBJECTIVE To conduct a quality markers(Q-Markers) study on the treatment of cough with Feire Zhike granules based on the “five principles” of Q-Markers, combined with mass spectrometry, network pharmacology and cobweb model. METHODS Identification of the main chemical components in Feire Zhike granules by liquid mass spectrometry. The TCMSP database was searched to collect the active ingredients and corresponding targets of 11 herbs in the prescription of Feire Zhike granules. The GeneCards database was also searched to collect genes encoding cough-related proteins, and a herb-component-target network map was constructed. Assigning effectiveness to active ingredients based on the number of target sites. Check the pharmacopoeia and literature to obtain the measurable components of each herb. Determine the monarchical, ministerial and adjuvant attribution of the original herbs in the compound formula based on the principle of compatibility. The literature and TCMSP database were searched to screen the original herb for specificity components. RESULTS A total of 43 chemical components in the Feire Zhike granules were identified to be “delivered” to the formulation based on UHPLC-Q-TOF-MS/MS. The 124 active compounds were obtained from the TCMSP database and acted on 120 targets, 93 of which were associated with coughing. There were 6 components in the pharmacopoeia that specify quantitative methods. A total of 26 endemic components were found in 11 herbs in the compound formula. In addition, the above components were ranked according to the compatibility rules of monarch and minister and the scoring rules of cobweb model. The top 10 components were wogonin, baicalein, licochalcone A, nobiletin, acacetin, hesperidin, liquiritin, tuberstemonine, β-sitosterol and galangin, respectively. CONCLUSION The above 10 components can be identified as Q-Markers of Feire Zhike granules.
