Effects of Shenfuhuang Formula （参附黄配方） on Potential Targets of Action in the Brain Tissue of Sepsis Model Mice：Transcriptomics-Based Exploration
10.13288/j.11-2166/r.2025.01.011
- VernacularTitle:基于转录组学探讨参附黄配方对脓毒症模型小鼠脑组织潜在作用靶点的影响
- Author:
Yuchen WANG
1
;
Xuerui WANG
2
;
Xiaolong XU
2
;
Jingxia ZHAO
2
;
Jiabo WANG
3
;
Yuan GAO
3
;
Weijun KONG
3
;
Qingquan LIU
2
Author Information
1. Beijing University of Chinese Medicine，Beijing，100029
2. Beijing Hospital of Traditional Chinese Medicine，Capital Medical University
3. School of Traditional Chinese Medicine，Capital Medical University
- Publication Type:Journal Article
- Keywords:
sepsis;
sepsis-associated encephalopathy;
transcriptomics;
Shenfuhuang Formula （参附黄配方）
- From:
Journal of Traditional Chinese Medicine
2025;66(1):65-70
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the possible mechanism of Shenfuhuang Formula （参附黄配方） in prevention and treatment of epsis-associated encephalopathy from the perspective of brain genomics. MethodsC57BL/6 mice were randomly divided into sham surgery group， sepsis group， and Shenfuhuang group， with 20 mice in each group. The sepsis group and Shenfuhuang group were induced to develop sepsis by cecal ligation and puncture （CLP） procedure. At 4 hours after modelling， Shenfuhuang group were gavaged with 2.5 g/（kg·d） of Shenfuhuang Formula， 0.5 ml each time， at 12 hours intervals， for a total of 4 times after modelling. Sepsis group and sham surgery group were given 0.5 ml of purified water orally. At 48 hours after modeling， the transcriptome sequencing was used to explore the differential gene expression in the effects of Shenfuhuang Formula on the brain regions of septic mice， and real-time PCR and ELISA were later used to further validate the differential gene and proteins expression. ResultsA total of 4605 genes were differentially expressed in Shenfuhuang group compared with sepsis group， of which 2353 genes were up-regulated and 2252 genes were down-regulated. According to the results of previous publications， six key genes were screened， including serine/threonine-protein kinase （Nek1）， myelin-associated glycoprotein （Mag）， endothelial cell-specific tyrosine kinase receptor （Tek）， a disintegrin and metalloproteinase with thrombospondin motifs 20 （Adamts20）， lymphocyte antigen 86 （Ly86）， and E3 ubiquitin-protein ligase （Traip）. Further genetic and protein validation revealed that， compared to the sham surgery group， the mRNA levels and corresponding protein levels of Nek1， Mag， Tek， Adamts20， Ly86， and Traip in the brain tissue of septic mice significantly reduced （P＜0.05）. In comparison to the sepsis group， Shenfuhuang group showed significantly increased mRNA levels and corresponding protein levels of Nek1， Mag， Tek， Adamts20， Ly86， and Traip （P＜0.05）. ConclusionThe potential therapeutic targets of Shenfuhuang Formula for treating sepsis-associated encephalopathy may be related to the Nek1， Mag， Tek， Adamts20， Ly86， and Traip genes and their encoded proteins.
