Study on the Protective Effect of Paeoniae Radix Alba on Angiopoiesis and Its Spectrum-effect Relationship
10.13748/j.cnki.issn1007-7693.20241498
- VernacularTitle:白芍对血管的保护作用及谱效关系研究
- Author:
WANG Fang
1
;
XU Pingcui
2
;
LIN Bingfeng
2
;
WANG Nani
2
Author Information
1. Zhejiang Chinese Medical University, Hangzhou 311400, China
2. Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310005, China
- Publication Type:Journal Article
- Keywords:
Paeoniae Radix Alba /fingerprints /vascular endothelial cell /zebrafish/ spectrum-effect relationship/ paeonin
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(14):1883-1891
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To establish the HPLC fingerprint of Paeoniae Radix Alba and investigate the spectrum-effect relationship between the fingerprint and the protection against vascular injury.METHODS The HPLC method was used to establish the fingerprint of Paeoniae Radix Alba. Lipopolysaccharide-induced human umbilical vein endothelial cells (HUVEC) and Tg(fli1:egfp) zebrafish models were used to evaluate in vivo and in vitro protective effects of 17 batches of Paeoniae Radix Alba extracts. Grey correlation degree and partial least squares method were used to analyze the spectrum-effect relationship between thefingerprint of Paeoniae Radix Alba and the protection effects.RESULTS The HPLC fingerprint of Paeoniae Radix Alba was constructed, and 11 common peaks were obtained, and the correlation was high, indicating that multiple components of Paeoniae Radix Alba may had synergistic effects on the protection of vascular endothelial cell activity. The correlation sequence with in vivo activitywasP6>P7>P10>P11>P9>P5>P3>P2>P8>P1>P4, and in vitro activity was P6>P4>P2>P3>P5>P1>P10>P9>P7>P11>P8, respectively. Peak 6 was identified as paeoniflorin. The regression coefficient of the partial least squares standard equation was positive, positively correlated with the protection of vascular endothelial cell activity and the vascular development in Tg(fli1:egfp) zebrafish, with VIP>1.0. Furthermore, the verification experiments of active ingredient showed that paeoniflorin could improve cell viability, migration, and tube formation, as well as promote vascular development in Tg(fli1:egfp) zebrafish in a dose-dependent manner. The above results indicated that paeoniflorin might be the major active component of Paeoniae Radix Alba for the protection effect on vascular endothelial cell.CONCLUSION The main active substance of Paeoniae Radix Alba in protecting against vascular inflammation and damage is paeoniflorin, which can provide reference for the quality control of medicinal materials.
