Protective effect of Shenbining granule on renal tissue of IgA nephropathy rats based on mitochondrial quality control system
- VernacularTitle:基于线粒体质量控制系统探讨肾必宁颗粒对IgA肾病大鼠的肾脏保护作用机制
- Author:
Yanmin FAN
1
,
2
;
Chundong SONG
1
,
2
;
Huiyuan SHI
1
,
2
;
Ke SONG
1
,
2
;
Chenchen CHEN
1
,
2
;
Xia ZHANG
1
,
2
;
Xianqing REN
1
,
2
;
Ying DING
1
,
2
;
Mo WANG
3
Author Information
1. Second Ward of Nephropathy Purpura，Pediatric Hospital，the First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450000，China
2. School of Pediatrics，Henan University of Chinese Medicine，Zhengzhou 450046，China
3. Dept. of Renal Immunology，Children’s Hospital of Chongqing Medical University，Chongqing 401122，China
- Publication Type:Journal Article
- Keywords:
Shenbining granule;
IgA nephropathy;
mitochondrial quality control system;
mitochondrial homeostasis;
autophagy
- From:
China Pharmacy
2024;35(24):2984-2989
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the renal protective mechanism of Shenbining granules on IgA nephropathy （IgAN） rats based on mitochondrial quality control system. METHODS IgAN rat model was established by the method of “bovine serum albumin+carbon tetrachloride+lipopolysaccharide”. The model rats were randomly divided into model group， prednisone acetate group （6.25 mg/kg）， Shenbining equal-dose group （4.1 g/kg） and Shenbining high-dose group （20.5 g/kg）. The normal rats were taken as the normal control group， with 12 rats in each group. Rats were given corresponding drugs or distilled water intragastrically in each group， once a day， for 4 consecutive weeks. After the last medication， the 24 h total urinary protein （24 h- UTP） and erythrocyte count in urine were determined， and the levels of serum creatinine （Scr）， blood urea nitrogen （BUN）， albumin （ALB） and alanine transaminase （ALT） were also detected. The histopathological changes in the kidneys and changes in IgA deposition in the mesangial area of the kidney were observed. mRNA and protein expression levels of PTEN-induced putative kinase 1 （PINK1）， E3 ubiquitin ligase（Parkin）， microtubule-associated protein 1 light chain-3 （LC3）， dynamin-related protein 1 （Drp1） and mitofusin 2 （Mfn2） were detected in the kidney tissues of rats. RESULTS Compared with model group， 24 h-UTP， urinary erythrocyte count， ALT， BUN and Scr levels， LC3-Ⅱ/LC3-Ⅰ mRNA ratio， mRNA and protein expressions of Drp1 were reduced significantly in prednisone acetate group， Shenbining equal-dose group and Shenbining high-dose group （P＜0.05）； ALB level， LC3-Ⅱ/LC3-Ⅰ protein ratio， mRNA and protein expressions of PINK1， Parkin and Mfn2 were increased significantly （P＜0.05）； the pathological morphology of kidney tissue in rats was significantly improved， and IgA deposition was significantly reduced. CONCLUSIONS Shenbining granule may reduce renal pathological injury in IgAN rats and protect renal function by activating the PINK1/Parkin pathway， enhancing mitochondrial autophagy， and correcting mitochondrial kinetic disorders.
