16S rDNA analysis of characteristic changes of intestinal flora in rat liver injury model by rifampicin
10.19405/j.cnki.issn1000-1492.2022.09.024
- Author:
Luming Yang
1
Author Information
1. School of Public Health , North China University of Technology, Tangshan 063200
- Publication Type:Journal Article
- Keywords:
anti⁃tuberculosis drug⁃induced liver injury;
rifampicin;
16S rDNA;
gut microbiot;
high⁃throughput se⁃ quencing
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1469-1474
- CountryChina
- Language:Chinese
-
Abstract:
Objective: 16 S rDNA sequencing technology was used to analyze the changes of intestinal flora in rats with anti-tuberculosis drug-induced liver injury(ADLI) induced by rifampicin(RFP).
Methods:Twenty-four male SD rats were randomly divided into control group(D0 group),RFP gavage group for 10 days(R10 group),and RFP gavage group for 28 days(R28 group).The fecal specimens of 4 rats in each group were selected by random number table method for 16 S rDNA sequencing.
Results : Compared with the D0 group, the α diversity and β diversity of the R10 and R28 groups both changed(allP<0.05),the abundance ofBacteroidetesincreased at the phylum level, the abundance ofFirmicutesdecreased(allP<0.05),and the abundance ofUnclassified＿f＿Prevotellaceae,Blautia,Prevotellaceae＿NK3B31＿group,Erysipelotrichaceae＿uCG-003,Fournierella,increased at the genuslevel,Lactobacillus,Romboutsia,Ruminococcaceae＿uCG-014 decreased in abundance(allP<0.05).
Conclusion : In the process of RFP-induced ADLI in rats, the number of intestinal flora decreased, the structure and composition changed, the beneficial bacteria decreased, and the pathogenic bacteria increased, but the diversity of the flora remained unchanged.
- Full text:2024122715120240491基于16S_rDNA测序技...鼠肝损伤中肠道菌群变化特征_杨璐铭.pdf
